EpCAM Rabbit Polyclonal Antibody
Catalog# ER1802-80
EpCAM Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
EpCAM Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human EpCAM aa 90-180.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Mouse colon tissue lysate, LOVO, MCF-7, rat epididymis tissue, human colon cancer tissue, mouse small intestine tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:50-1:200
Target
Function
The epithelial cell adhesion molecule Ep-CAM, which is also designated tumor-associated calcium signal transducer 1 and MK-1, is a monomeric membrane glycoprotein that is expressed in most normal human epithelium and carcinomas. The human Ep-CAM gene encodes a 314 amino acid protein that is expressed as two forms, a major form and a minor form, which are reduced upon treatment with the amino-glycosylation inhibitor tunicamycin. Ep-CAM is overexpressed in a variety of carcinomas and is, therefore, a potential target for the visualization and therapy of human solid tumors. Ep-CAM contains an extracellular domain containing two epidermal growth factor-like repeats, followed by a cysteine poor region, which is necessary for the adhesion properties of the molecule.
Background References
1. Muenz M et al. The carcinoma-associated antigen EpCAM upregulates c-myc and induces cell proliferation. Oncogene 23:5748-5758 (2004).
2. Muenz M et al. The tumour-associated antigen EpCAM upregulates the fatty acid binding protein E-FABP. Cancer Lett 225:151-157 (2005).
Sequence Similarity
Belongs to the EPCAM family.
Tissue Specificity
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post-translational Modification
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Subcellular Location
Cell junction, Plasma membrane.
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
Cell surface glycoprotein Trop 2 antibody
ExpandImages
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Western blot analysis of EpCAM on Mouse colon tissue lysate with Rabbit anti-EpCAM antibody (ER1802-80) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 46 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-80, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35 kDa
Observed band size: 35/38 kDa -
Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-EpCAM antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EpCAM antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-EpCAM antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Citation
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Rapid Detection and Purification of Extracellular Vesicles for Hepatocellular Carcinoma Screening Using a Plasmonic Metasurface Integrated with the Kolmogorov–Arnold Network
Journal: ACS Applied Nano Materials
DOI: 10.1021/acsnano.5c11740
IF: 16
Application: WB
Reactivity:
Publish date: 2025 Dec
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