EpCAM Recombinant Rabbit Monoclonal Antibody
Catalog# IRS254RB
EpCAM Recombinant Rabbit Monoclonal Antibody
-
mIHC
-
Human
-
unconjugated
Overview
Product Name
EpCAM Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 35 kDa
Positive Control
Human colon tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
EPCAM is a carcinoma-associated antigen and belongs to a family which includes at least 2 type I membrane proteins. The EPCAM protein has a role in embryonic stem cells proliferation and differentiation. EPCAM is used as a target for immunotherapy treatment of human carcinomas. EPCAM is expressed on most normal epithelial cells and gastrointestinal carcinomas and acts as a homotypic calcium-independent cell adhesion molecule. Epithelial cell adhesion molecules (EPCAM) can act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for supplying immunological barrier as a first line of defense against mucosal infection. EPCAM gene mutations result in congenital tufting enteropathy.
Background References
1. Guye P et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016)
2. Holditch SJ et al. B-Type Natriuretic Peptide Deletion Leads to Progressive Hypertension, Associated Organ Damage, and Reduced Survival: Novel Model for Human Hypertension. Hypertension 66:199-210 (2015).
Subcellular Location
Lateral cell membrane, Cell junction.
UNIPROT
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
ExpandImages
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mIHC analysis of human colon tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-EpCAM antibody (IRS254RB) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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