iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Catalog# HA720185F
iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
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FC
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IF-Cell
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Human
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iFluor™ 647
Overview
Product Name
iFluor™ 647 Conjugated EpCAM Recombinant Rabbit Monoclonal Antibody [PS01-69]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide.
Species Reactivity
Human
Validated Applications
FC, IF-Cell
Target Molecular Weight
Predicted band size: 35 kDa
Positive Control
HT-29.
Conjugation
iFluor™ 647
Clone Number
PS01-69
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| FC | IF-Cell | |
|---|---|---|
| human |
|
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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1:1,000
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IF-Cell
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1:500
Target
Function
EPCAM is a carcinoma-associated antigen and belongs to a family which includes at least 2 type I membrane proteins. The EPCAM protein has a role in embryonic stem cells proliferation and differentiation. EPCAM is used as a target for immunotherapy treatment of human carcinomas. EPCAM is expressed on most normal epithelial cells and gastrointestinal carcinomas and acts as a homotypic calcium-independent cell adhesion molecule. Epithelial cell adhesion molecules (EPCAM) can act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for supplying immunological barrier as a first line of defense against mucosal infection. EPCAM gene mutations result in congenital tufting enteropathy.
Background References
1. Li D et al. EpCAM-targeting CAR-T cell immunotherapy is safe and efficacious for epithelial tumors. Sci Adv. 2023 Dec
2. Li F et al. EpCAM-Targeting Aptamer Radiotracer for Tumor-Specific PET Imaging. Bioconjug Chem. 2021 Jun
Sequence Similarity
Belongs to the EPCAM family.
Tissue Specificity
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post-translational Modification
Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Subcellular Location
Lateral cell membrane, Cell junction.
UNIPROT
Synonyms
17 1A antibody
323/A3 antibody
Adenocarcinoma associated antigen antibody
Adenocarcinoma-associated antigen antibody
Antigen identified by monoclonal antibody
AUA1 antibody
AUA1 antibody
CD326 antibody
CD326 antigen antibody
Cell surface glycoprotein Trop 1 antibody
ExpandImages
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Flow cytometric analysis of HT-29 cells labeling EpCAM (HA720185F, iFluor™ 647).
Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with EpCAM (HA720185F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Immunocytochemistry analysis of HT-29 cells labeling EpCAM with Rabbit anti-EpCAM antibody (HA720185F) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-EpCAM antibody (HA720185F, iFluor™ 647) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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