Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH14-91]
Catalog# HA723693
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH14-91]
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WB
-
IF-Cell
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IP
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Human
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HA751535
不含抗保成分
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unconjugated
Overview
Product Name
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH14-91]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Eph receptor B2 aa 1-543.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IP
Molecular Weight
Predicted band size: 117 kDa
Positive Control
COLO205 cell lysate, Saos-2 cell lysate, SW480 cell lysate, COLO205.
Conjugation
unconjugated
Clone Number
PSH14-91
Reactivity Data
| WB | IF-Cell | IP | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
||
| Rat |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
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IF-Cell
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1:100
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IP
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1-2μg/sample
Target
Function
Ephrin type-B receptor 2 is a protein that in humans is encoded by the EPHB2 gene. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Background References
1. Liu W et al. The Roles of EphB2 in Cancer. Front Cell Dev Biol. 2022 Feb
2. Xiao Y et al.Hepatocytes demarcated by EphB2 contribute to the progression of nonalcoholic steatohepatitis. Sci Transl Med. 2023 Feb
Subcellular Location
Cell membrane, Cell projection, axon, dendrite.
UNIPROT
Synonyms
cek5 antibody
Developmentally regulated EPH related tyrosine kinase antibody
DRT antibody
EK5 antibody
ELK related protein tyrosine kinase antibody
Eph receptor B2 antibody
EPH tyrosine kinase 3 antibody
EPH-like kinase 5 antibody
EPHB2 antibody
EPHB2_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/5,000 dilution.
Lane 1: COLO205 cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: Saos-2 cell lysate
Lane 4: SW480 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 130 kDa
Exposure time: Lane 1-3: 20 seconds; Lane 4: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723693) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of COLO205 (positive) and K-562 (negative) labeling Eph receptor B2 with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Eph receptor B2 antibody (HA723693) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Eph receptor B2 was immunoprecipitated from 0.2 mg COLO205 cell lysate with HA723693 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723693 at 1/5,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: COLO205 cell lysate (input)
Lane 2: HA723693 IP in COLO205 cell lysate
Lane 3: Rabbit IgG instead of HA723693 in COLO205 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
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Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74] - BSA and Azide free
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Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
