Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74]

Overview

Product Name

Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human Eph receptor B2 aa 1-543.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-Fr, IP

Target Molecular Weight

Predicted band size: 117 kDa

Positive Control

COLO205 (Human colon adenocarcinoma cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate, Saos-2 (Human osteosarcoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, 4T1 (Mouse breast cancer cells) cell lysate, Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate.

Conjugation

unconjugated

Clone Number

PSH23-74

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Lot Concentration Lookup

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

WB
1:5,000

IHC-Fr
1:500

IP
1-2μg/sample

Target

Function

Ephrin type-B receptor 2 is a protein that in humans is encoded by the EPHB2 gene. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.

Background References

1. Liu W et al. The Roles of EphB2 in Cancer. Front Cell Dev Biol. 2022 Feb

2. Xiao Y et al.Hepatocytes demarcated by EphB2 contribute to the progression of nonalcoholic steatohepatitis. Sci Transl Med. 2023 Feb

Subcellular Location

Cell membrane, Cell projection, axon, dendrite.

UNIPROT

SwissProt: P29323 Human

SwissProt: P54763 Mouse

Entrez Gene: 313633 Rat

Synonyms

cek5 antibody

Developmentally regulated EPH related tyrosine kinase antibody

DRT antibody

EK5 antibody

ELK related protein tyrosine kinase antibody

Eph receptor B2 antibody

EPH tyrosine kinase 3 antibody

EPH-like kinase 5 antibody

EPHB2 antibody

EPHB2_HUMAN antibody

Expand

Images

☑ Relative expression (RE)

Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody (HA751909) at 1/5,000 dilution.

Lane 1: COLO205 (Human colon adenocarcinoma cells) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 3: Saos-2 (Human osteosarcoma cells) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 5: 4T1 (Mouse breast cancer cells) cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Mouse colon tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: Rat colon tissue lysate

Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane.
Exposure time: 3 minutes; ECL: K1801

K-562 is a negative control (PMID : 18713744, PMID : 21508957).

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 117 kDa
Observed band size: 130 kDa
Application: Immunofluorescence (IHC-Fr)

Species: Mouse
Tissue: Colon
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751909, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Application: Immunofluorescence (IHC-Fr)

Species: Rat
Tissue: Colon
Sample: Frozen section

Antigen retrieval: Not required

Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751909, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.
Immunoprecipitation (IP)

Eph receptor B2 was immunoprecipitated in 0.2 mg COLO205 (Human colon adenocarcinoma cells) cell lysate with HA751909 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751909 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: COLO205 cell lysate (input)
Lane 2: HA751909 IP in COLO205 cell lysate
Lane 3: Rabbit IgG instead of HA751909 in COLO205 cell lysate

Exposure time: 2 minutes 6 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature

Primary dilution: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature

Predicted band size: 117 kDa
Observed band size: 130 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"