Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74] - BSA and Azide free
Catalog# HA751909
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74] - BSA and Azide free
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WB
-
IHC-Fr
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IP
-
Human
-
Mouse
-
Rat
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HA724397
含抗保成分
-
unconjugated
Overview
Product Name
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74] - BSA and Azide free
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Eph receptor B2 aa 1-543.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-Fr, IP
Molecular Weight
Predicted band size: 117 kDa
Positive Control
COLO205 (Human colon adenocarcinoma cells) cell lysate, K-562 (Human chronic myelogenous leukemia cells) cell lysate, Saos-2 (Human osteosarcoma cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, 4T1 (Mouse breast cancer cells) cell lysate, Mouse brain tissue lysate, Mouse colon tissue lysate, Rat brain tissue lysate, Rat colon tissue lysate.
Conjugation
unconjugated
Clone Number
PSH23-74
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
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IHC-Fr
-
1:500
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IP
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1-2μg/sample
Target
Function
Ephrin type-B receptor 2 is a protein that in humans is encoded by the EPHB2 gene. Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members.
Background References
1. Liu W et al. The Roles of EphB2 in Cancer. Front Cell Dev Biol. 2022 Feb
2. Xiao Y et al.Hepatocytes demarcated by EphB2 contribute to the progression of nonalcoholic steatohepatitis. Sci Transl Med. 2023 Feb
Subcellular Location
Cell membrane, Cell projection, axon, dendrite.
Synonyms
cek5 antibody
Developmentally regulated EPH related tyrosine kinase antibody
DRT antibody
EK5 antibody
ELK related protein tyrosine kinase antibody
Eph receptor B2 antibody
EPH tyrosine kinase 3 antibody
EPH-like kinase 5 antibody
EPHB2 antibody
EPHB2_HUMAN antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of Eph receptor B2 on different lysates with Rabbit anti-Eph receptor B2 antibody (HA751909) at 1/5,000 dilution.
Lane 1: COLO205 (Human colon adenocarcinoma cells) cell lysate
Lane 2: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 3: Saos-2 (Human osteosarcoma cells) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 5: 4T1 (Mouse breast cancer cells) cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Mouse colon tissue lysate
Lane 8: Rat brain tissue lysate
Lane 9: Rat colon tissue lysate
Cell lysates/proteins at 20 µg/Lane. Tissue lysates/proteins at 40 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
K-562 is a negative control (PMID : 18713744, PMID : 21508957).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 117 kDa
Observed band size: 130 kDa -
Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Colon
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751909, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Application: Immunofluorescence (IHC-Fr)
Species: Rat
Tissue: Colon
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
Primary antibody: HA751909, 1/500, overnight at 4°C.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature. -
Immunoprecipitation (IP)
Eph receptor B2 was immunoprecipitated in 0.2 mg COLO205 (Human colon adenocarcinoma cells) cell lysate with HA751909 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA751909 at 1/5,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: COLO205 cell lysate (input)
Lane 2: HA751909 IP in COLO205 cell lysate
Lane 3: Rabbit IgG instead of HA751909 in COLO205 cell lysate
Exposure time: 2 minutes 6 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA751909, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 117 kDa
Observed band size: 130 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH14-91]
Application: WB,IF-Cell,IP
Reactivity: Human
Conjugate: unconjugated
Eph receptor B2 Recombinant Rabbit Monoclonal Antibody [PSH23-74]
Application: WB,IHC-Fr,IP
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
