FADD Recombinant Rabbit Monoclonal Antibody [JE45-25]
Catalog# HA721353
FADD Recombinant Rabbit Monoclonal Antibody [JE45-25]
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
unconjugated
Overview
Product Name
FADD Recombinant Rabbit Monoclonal Antibody [JE45-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FADD aa 1-170 / 208.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Molecular Weight
Predicted band size: 23 kDa
Positive Control
A431 cell lysate, K-562 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, SK-Br-3 cell lysate, SW480 cell lysate, THP-1 cell lysate, A549 cell lysate, HepG2 cell lysate, SW480, human stomach tissue.
Conjugation
unconjugated
Clone Number
JE45-25
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
IF-Cell
-
1:50
Target
Function
FAS-associated death domain protein, also called MORT1, is encoded by the FADD gene on the 11q13.3 region of chromosome 11 in humans. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development.
Background References
1. Schwarzer R et al. FADD and Caspase-8 Regulate Gut Homeostasis and Inflammation by Controlling MLKL- and GSDMD-Mediated Death of Intestinal Epithelial Cells. Immunity. 2020 Jun
2. Rodriguez DA et al. Caspase-8 and FADD prevent spontaneous ZBP1 expression and necroptosis. Proc Natl Acad Sci U S A. 2022 Oct
Subcellular Location
Cytoplasm, cytosol, neuron projection, nucleus, plasma membrane, ripoptosome.
UNIPROT
Synonyms
FADD antibody
FADD protein antibody
FADD_HUMAN antibody
Fas (TNFRSF6) associated via death domain antibody
Fas associated via death domain antibody
Fas associating death domain containing protein antibody
Fas associating protein antibody
Fas associating protein with death domain antibody
Fas TNFRSF6 associated via death domain antibody
FAS-associated death domain protein antibody
ExpandImages
-
Western blot analysis of FADD on different lysates with Rabbit anti-FADD antibody (HA721353) at 1/1,000 dilution.
Lane 1: A431 cell lysate (10 µg/Lane)
Lane 2: K-562 cell lysate (10 µg/Lane)
Lane 3: Jurkat cell lysate (10 µg/Lane)
Lane 4: MCF7 cell lysate (10 µg/Lane)
Lane 5: HeLa cell lysate (10 µg/Lane)
Lane 6: SK-Br-3 cell lysate (10 µg/Lane)
Lane 7: SW480 cell lysate (10 µg/Lane)
Lane 8: THP-1 cell lysate (10 µg/Lane)
Lane 9: A549 cell lysate (10 µg/Lane)
Lane 10: HepG2 cell lysate (10 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 90 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721353) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SW480 cells labeling FADD with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-FADD antibody (HA721353) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-FADD antibody (HA721353) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721353) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
FADD Recombinant Rabbit Monoclonal Antibody [PSH07-78]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
FADD Recombinant Rabbit Monoclonal Antibody [PSH01-13] - BSA and Azide free
Application: WB,IF-Cell,FC
Reactivity: Human
Conjugate: unconjugated
