FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-61] - BSA and Azide free

This data was developed using HA724014, the same antibody clone in a different buffer formulation.
Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA724014) at 1/2,000 dilution.

Lane 1: U-87 MG cell lysate
Lane 2: U-2 OS cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 17 kDa
Observed band size: 17 kDa

Exposure time: 1 minute 20 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724014) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA724014, the same antibody clone in a different buffer formulation.
Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA724014) at 1/5,000 dilution.

Lane 1: Neuro-2a cell lysate (30 µg/Lane)
Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
Lane 3: Mouse heart tissue lysate (30 µg/Lane)
Lane 4: Rat heart tissue lysate (30 µg/Lane)

Predicted band size: 17 kDa
Observed band size: 16 kDa

Exposure time: 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724014) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

This data was developed using HA724014, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of U-87 MG cells labeling FGF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA724014, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

This data was developed using HA724014, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Neuro-2a cells labeling FGF1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA724014, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).