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FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-61]

Usd: 385

Specification

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Catalog# HA724014

FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-61]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

FGF1 Recombinant Rabbit Monoclonal Antibody [PSH18-61]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FGF1 aa 1-155.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 18 kDa

Positive Control

U-87 MG cell lysate, U-2 OS cell lysate, Neuro-2a cell lysate, Mouse kidney tissue lysate, Mouse heart tissue lysate, Rat heart tissue lysate, U-87 MG, Neuro-2a.

Conjugation

unconjugated

Clone Number

PSH18-61

Reactivity Data

WB FC
Human
Tested Tested
Tested Tested
Mouse
Tested Tested
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000-1:5,000

  • FC

  • 1:1,000

Target

Function

Fibroblast growth factor 1, (FGF-1) also known as acidic fibroblast growth factor (aFGF), is a growth factor and signaling protein encoded by the FGF1 gene. It is synthesized as a 155 amino acid polypeptide, whose mature form is a non-glycosylated 17-18 kDa protein. Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated acidic FGF, Heparin-binding growth factor-1, and Endothelial cell growth factor-1. Gene sequencing revealed that this group was actually the same growth factor and that FGF1 was a member of a family of FGF proteins.

Background References

1. Sancar G et al. FGF1 and insulin control lipolysis by convergent pathways. Cell Metab. 2022 Jan

2. Wang D et al. FGF1(deltaHBS) prevents diabetic cardiomyopathy by maintaining mitochondrial homeostasis and reducing oxidative stress via AMPK/Nur77 suppression. Signal Transduct Target Ther. 2021 Mar

Subcellular Location

Secreted, Cytoplasm, cell cortex, cytosol, Nucleus.

UNIPROT

SwissProt: P05230 Human

SwissProt: P61148 Mouse

SwissProt: P61149 Rat

Synonyms

Acidic fibroblast growth factor antibody

aFGF antibody

Beta endothelial cell growth factor antibody

Beta-endothelial cell growth factor antibody

ECGF antibody

ECGF beta antibody

ECGF-beta antibody

ECGFA antibody

ECGFB antibody

Endothelial Cell Growth Factor alpha antibody

Expand

Images

  • Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>) at 1/2,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: U-2 OS cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa<br /><br />Exposure time: 1 minute 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA724014) at 1/2,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: U-2 OS cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

    Exposure time: 1 minute 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724014) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>) at 1/5,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate (30 µg/Lane)<br />Lane 2: Mouse kidney tissue lysate (30 µg/Lane)<br />Lane 3: Mouse heart tissue lysate (30 µg/Lane)<br />Lane 4: Rat heart tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 17 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FGF1 on different lysates with Rabbit anti-FGF1 antibody (HA724014) at 1/5,000 dilution.

    Lane 1: Neuro-2a cell lysate (30 µg/Lane)
    Lane 2: Mouse kidney tissue lysate (30 µg/Lane)
    Lane 3: Mouse heart tissue lysate (30 µg/Lane)
    Lane 4: Rat heart tissue lysate (30 µg/Lane)

    Predicted band size: 17 kDa
    Observed band size: 16 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724014) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of U-87 MG cells labeling FGF1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of U-87 MG cells labeling FGF1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA724014, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Flow cytometric analysis of Neuro-2a cells labeling FGF1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA724014" style="font-weight: bold;text-decoration: underline;">HA724014</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Neuro-2a cells labeling FGF1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA724014, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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