FGF2 Recombinant Rabbit Monoclonal Antibody [PSH22-75]
Catalog# HA724320
FGF2 Recombinant Rabbit Monoclonal Antibody [PSH22-75]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
FGF2 Recombinant Rabbit Monoclonal Antibody [PSH22-75]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FGF-2 aa 113-288.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Molecular Weight
Predicted band size: 31 kDa
Positive Control
K-562 cell lysate, HeLa cell lysate, U-87 MG cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
PSH22-75
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:10,000
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FC
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1:1,000
Target
Function
FGF2, also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins, themselves a family of closely related molecules. Fibroblast growth factor protein was first purified in 1975; soon thereafter three variants were isolated: 'basic FGF' (FGF2); Heparin-binding growth factor-2; and Endothelial cell growth factor-2. Gene sequencing revealed that this group is the same FGF2 protein and is a member of a family of FGF proteins. Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
Background References
1. Park IS et al. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS One 10:e0122776 (2015).
2. Rotschafer JH et al. Modulation of neural stem/progenitor cell proliferation during experimental Herpes Simplex encephalitis is mediated by differential FGF-2 expression in the adult brain. Neurobiol Dis 58:144-55 (2013).
Subcellular Location
Secreted, Nucleus.
UNIPROT
Synonyms
Basic fibroblast growth factor antibody
Basic fibroblast growth factor bFGF antibody
BFGF antibody
FGF 2 antibody
FGF B antibody
FGF-2 antibody
Fgf2 antibody
FGF2 basic antibody
FGF2_HUMAN antibody
FGFB antibody
ExpandImages
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Western blot analysis of FGF2 on different lysates with Rabbit anti-FGF2 antibody (HA724320) at 1/2,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: U-87 MG cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 18/22/24 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724320) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling FGF2 with Rabbit anti-FGF2 antibody (HA724320) at 1/1,0000 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-FGF2 antibody (HA724320) at 1/1,0000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of HeLa cells labeling FGF2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA724320, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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