Human FGF2 Recombinant Rabbit Monoclonal Antibody [PSH02-09] - BSA and Azide free (Detector)
Overview
Product Name
Human FGF2 Recombinant Rabbit Monoclonal Antibody [PSH02-09] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human FGF-2 aa 143-288 (P09038).
Species Reactivity
Human
Validated Applications
ELISA(Det)
Molecular Weight
Predicted band size: 30 kDa
Positive Control
Recombinant Human FGF-2 protein (HA210834).
Conjugation
unconjugated
Clone Number
PSH02-09
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-08] to Human FGF-2 (Detector) (HA721780) and recombinant Human FGF-2 protein (HA210834) as the standard. The reference range value is 2.74-2,000 pg/mL.
Target
Function
FGF2, also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene. It binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins, themselves a family of closely related molecules. Fibroblast growth factor protein was first purified in 1975; soon thereafter three variants were isolated: 'basic FGF' (FGF2); Heparin-binding growth factor-2; and Endothelial cell growth factor-2. Gene sequencing revealed that this group is the same FGF2 protein and is a member of a family of FGF proteins. Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide.
Background References
1. Park IS et al. Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation. PLoS One 10:e0122776 (2015).
2. Rotschafer JH et al. Modulation of neural stem/progenitor cell proliferation during experimental Herpes Simplex encephalitis is mediated by differential FGF-2 expression in the adult brain. Neurobiol Dis 58:144-55 (2013).
Subcellular Location
Secreted, Nucleus.
UNIPROT
Synonyms
Basic fibroblast growth factor antibody
Basic fibroblast growth factor bFGF antibody
BFGF antibody
FGF 2 antibody
FGF B antibody
FGF-2 antibody
Fgf2 antibody
FGF2 basic antibody
FGF2_HUMAN antibody
FGFB antibody
ExpandImages
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![Sandwich ELISA analysis of Human FGF-2 matched pair antibodies.<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721780" style="font-weight: bold;text-decoration: underline;">HA721780</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human FGF-2 protein (<a href="/products/HA210834" style="font-weight: bold;text-decoration: underline;">HA210834</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-09]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721781_1.jpg?v=20260509231355)
Sandwich ELISA analysis of Human FGF-2 matched pair antibodies.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721780) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Human FGF-2 protein (HA210834) starting from 2000 pg/ml to 0 pg/ml and detect antibody [PSH02-09]-Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Interpolated concentrations of native FGF-2 in Hela cell extract and U-87 MG cell extract:
The concentrations of FGF-2 were measured in duplicates, interpolated from the FGF-2 standard curve and corrected for sample dilution. Undiluted samples are Hela cell extract 0.1ug/ml and U-87 MG cell extract 0.1ug/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF-2 concentration was determined to be 2,106 pg/ml in Hela cell extract and 1,065 pg/ml in U-87 MG cell extract 0.1ug/ml.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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