FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
Catalog# HA723197
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
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WB
-
IP
-
Human
-
Mouse
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HA751347
不含抗保成分
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unconjugated
Overview
Product Name
FOXM1 Recombinant Rabbit Monoclonal Antibody [PSH10-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FOXM1 aa 1-400.
Species Reactivity
Human, Mouse
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 84 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, HT-29 cell lysate, LO2 cell lysate, Mouse testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH10-30
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IP
-
1-2μg/sample
Target
Function
Forkhead box protein M1 is a protein that in humans is encoded by the FOXM1 gene. The protein encoded by this gene is a member of the FOX family of transcription factors. FOXM1 is known to play a key role in cell cycle progression where endogenous FOXM1 expression peaks at S and G2/M phases. FOXM1-null mouse embryos were neonatal lethal as a result of the development of polyploid cardiomyocytes and hepatocytes, highlighting the role of FOXM1 in mitotic division. More recently a study using transgenic/knockout mouse embryonic fibroblasts and human osteosarcoma cells (U2OS) has shown that FOXM1 regulates expression of a large array of G2/M-specific genes, such as Plk1, cyclin B2, Nek2 and CENPF, and plays an important role in maintenance of chromosomal segregation and genomic stability.
Background References
1. Khan MA et al. FOXM1: A small fox that makes more tracks for cancer progression and metastasis. Semin Cancer Biol. 2023 Jul
2. Zhang Z et al. FOXM1: Functional Roles of FOXM1 in Non-Malignant Diseases. Biomolecules. 2023 May
Subcellular Location
Nucleus.
Synonyms
FKHL16 antibody
Forkhead box M1 antibody
Forkhead box protein M1 antibody
forkhead like 16 antibody
Forkhead-related protein FKHL16 antibody
FOX M1 antibody
Foxm1 antibody
FOXM1_HUMAN antibody
FOXM1B antibody
Hepatocyte nuclear factor 3 forkhead homolog 11 antibody
ExpandImages
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Western blot analysis of FOXM1 on different lysates with Rabbit anti-FOXM1 antibody (HA723197) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: HT-29 cell lysate (20 µg/Lane)
Lane 4: LO2 cell lysate (20 µg/Lane)
Lane 5: Mouse testis tissue lysate (40 µg/Lane)
Predicted band size: 84 kDa
Observed band size: 110 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723197) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
FOXM1 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA723197 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA723197 at 1/1,000 dilution. Mouse Anti-Rabbit IgG kappa light chain secondary antibody (M1208-2) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA723197 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA723197 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 4 seconds; ECL: K1801
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"