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Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [SC0620]

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Specification

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Catalog# ET1610-78

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [SC0620]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

  • IHC-Fr

Reactivity

  • Human

  • Mouse

  • Zebrafish

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [SC0620]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human FTH1 aa 58-99 / 183.

Species Reactivity

Human, Mouse, Zebrafish, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC, IHC-Fr

Molecular Weight

Predicted band size: 21 kDa

Positive Control

293T cell lysate, RAW264.7 cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, SGC-7901, Jurkat, human spleen tissue, mouse liver tissue, rat liver tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

SC0620

RRID

AB_3069963

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:20,000

  • IF-Cell

  • 1:100

  • IF-Tissue

  • 1:200

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

  • IHC-Fr

  • 1:500

Target

Function

Ferritin is a universal intracellular protein that stores iron and releases it in a controlled fashion. The protein is produced by almost all living organisms, including archaea, bacteria, algae, higher plants, and animals. It is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is found in most tissues as a cytosolic protein, but small amounts are secreted into the serum where it functions as an iron carrier. Plasma ferritin is also an indirect marker of the total amount of iron stored in the body; hence, serum ferritin is used as a diagnostic test for iron-deficiency anemia. Aggregated ferritin transforms into a toxic form of iron called hemosiderin. Ferritin is a globular protein complex consisting of 24 protein subunits forming a hollow nanocage with multiple metal–protein interactions. Ferritin that is not combined with iron is called apoferritin.

Background References

1. Ben-Othman R et al. Leishmania-mediated inhibition of iron export promotes parasite replication in macrophages. PLoS Pathog 10:e1003901 (2014).

2. Chin D et al. Curcumin may impair iron status when fed to mice for six months. Redox Biol 2:563-9 (2014).

Sequence Similarity

Belongs to the ferritin family.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P02794 Human

SwissProt: P09528 Mouse

SwissProt: P19132 Rat

Synonyms

Cell proliferation-inducing gene 15 protein antibody

Ferritin H subunit antibody

Ferritin heavy chain antibody

Ferritin heavy polypeptide 1 antibody

Ferritin L subunit antibody

Ferritin, heavy polypeptide antibody

FRIH_HUMAN antibody

FTH antibody

FTH1 antibody

FTL antibody

Images

  • Western blot analysis of Ferritin Heavy Chain on different lysates with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.<br /><br />Lane 1: 293T cell lysate (30 µg/Lane)<br />Lane 2: RAW264.7 cell lysate (15 µg/Lane)<br />Lane 3: Mouse liver tissue lysate (15 µg/Lane)<br />Lane 4: Mouse brain tissue lysate (15 µg/Lane)<br />Lane 5: Rat liver tissue lysate (15 µg/Lane)<br />Lane 6: Rat brain tissue lysate (30 µg/Lane)<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa<br /><br />Exposure time: 3 minutes 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ferritin Heavy Chain on different lysates with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.

    Lane 1: 293T cell lysate (30 µg/Lane)
    Lane 2: RAW264.7 cell lysate (15 µg/Lane)
    Lane 3: Mouse liver tissue lysate (15 µg/Lane)
    Lane 4: Mouse brain tissue lysate (15 µg/Lane)
    Lane 5: Rat liver tissue lysate (15 µg/Lane)
    Lane 6: Rat brain tissue lysate (30 µg/Lane)

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

    Exposure time: 3 minutes 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-78) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of Ferritin Heavy Chain with anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/5,000 dilution.<br />Lane 1/2: Wild-type SH-SY5Y whole cell lysate (10 µg).<br />Lane 3/4: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg).<br />Lane 5/6: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg).<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, <a href="/products/ET1601-4" style="font-weight: bold;text-decoration: underline;">ET1601-4</a>, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of Ferritin Heavy Chain with anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/5,000 dilution.
    Lane 1/2: Wild-type SH-SY5Y whole cell lysate (10 µg).
    Lane 3/4: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg).
    Lane 5/6: Ferritin Heavy Chain fragment 1 knockout SH-SY5Y whole cell lysate (10 µg).

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-78, 1/5,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1/200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SGC-7901 cells labeling Ferritin Heavy Chain with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/100 dilution.<br /><br />Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SGC-7901 cells labeling Ferritin Heavy Chain with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution.

    Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of Jurkat cells labeling Ferritin Heavy Chain with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling Ferritin Heavy Chain with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: liver<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: liver

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-78) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-78) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-78) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-78) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of Jurkat cells labeling Ferritin Heavy Chain.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling Ferritin Heavy Chain.

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1610-78, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of Ferritin Heavy Chain on different lysates with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/10,000 dilution.<br /><br />Lane 1: FTL recombinant protein, 20ng/Lane<br />Lane 2: FTH1 recombinant protein, 20ng/Lane<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-78" style="font-weight: bold;text-decoration: underline;">ET1610-78</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Ferritin Heavy Chain on different lysates with Rabbit anti-Ferritin Heavy Chain antibody (ET1610-78) at 1/10,000 dilution.

    Lane 1: FTL recombinant protein, 20ng/Lane
    Lane 2: FTH1 recombinant protein, 20ng/Lane

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-78) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

Application: IHC-P

Reactivity: Human

Conjugate: unconjugated