Skip to content

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1705-55

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

Application

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [JM22-36]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within C-terminal human Ferritin Heavy Chain.

Species Reactivity

Human

Validated Applications

IHC-P

Molecular Weight

Predicted band size: 21 kDa

Positive Control

Human kidney tissue, human liver tissue, human placenta tissue.

Conjugation

unconjugated

Clone Number

JM22-36

RRID

AB_3070583

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:200-1:1,000

Target

Function

Mammalian ferritins consist of 24 subunits made up of two types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of FeII, whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of FeIII. The most prominent role of mamma-lian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed; and oxidative stress, an important factor in the development of aging-related cataracts.

Background References

1. Vannucci L et al. In Vivo Targeting of Cutaneous Melanoma Using an Melanoma Stimulating Hormone-Engineered Human Protein Cage with Fluorophore and Magnetic Resonance Imaging Tracers. J Biomed Nanotechnol 11:81-92 (2015).

2. Yang H et al. Upregulation of mitochondrial ferritin by proinflammatory cytokines: implications for a role in Alzheimer\'s disease. J Alzheimers Dis 45:797-811 (2015).

Sequence Similarity

Belongs to the ferritin family.

Tissue Specificity

Expressed in the liver.

Subcellular Location

Cytoplasm. Nucleus.

UNIPROT

SwissProt: P02794 Human

Synonyms

Apoferritin antibody

Cell proliferation inducing gene 15 protein antibody

Cell proliferation-inducing gene 15 protein antibody

F HC antibody

Ferritin H subunit antibody

Ferritin heavy chain antibody

Ferritin heavy polypeptide 1 antibody

FHC antibody

FRIH antibody

FRIH_HUMAN antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1705-55) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Ferritin Heavy Chain antibody (ET1705-55) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1705-55" style="font-weight: bold;text-decoration: underline;">ET1705-55</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Ferritin Heavy Chain antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

Ferritin Heavy Chain Recombinant Rabbit Monoclonal Antibody [SC0620]

Application: WB,IF-Cell,IF-Tissue,IHC-P,FC,IHC-Fr

Reactivity: Human,Mouse,Zebrafish,Rat

Conjugate: unconjugated