Fibulin 5 Rabbit Polyclonal Antibody
Catalog# ER1802-95
Fibulin 5 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Fibulin 5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within Human Fibulin aa 203-378 / 448.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, FC
Target Molecular Weight
50 kDa
Positive Control
Human skin tissue, JAR, SiHa, SKOV-3, rat testis tissue, mouse ovary tissue, Hela.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IF-Cell
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1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
Fibulin proteins contribute to normal development of elastic fiber systems in various types of organs that require elasticity, such as vasculature, lung and skin. Fibulin-5 (EVEC, UP50, DANCE) is an integrin-binding extracellular matrix protein that mediates endothelial cell adhesion. Fibulin-5 is also a calcium-dependent elastin-binding protein that scaffolds cells to elastic fibers, thereby preventing elastinopathy in the skin, lung, and vasculature. The Arg-Gly-Asp (RGD) motif in Fibulin-5 interacts with cell surface integrins avb3, avb5 and a9b1, serves as an anchorage for elastic fibers to cells, and promotes organization of elastic fibers. The human Fibulin-5 gene maps to chromosome 14q32.12 and encodes a 488 amino acid protein.
Background References
暂无
Sequence Similarity
Belongs to the fibulin family.
Tissue Specificity
Expressed in skin fibroblasts (at protein level)(PubMed:17035250). Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.
Post-translational Modification
N-glycosylated.
Subcellular Location
Extracellular matrix. Secreted.
Synonyms
ADCL2 antibody
ARCL1A antibody
ARMD3 antibody
Dance antibody
Developmental arteries and neural crest EGF like protein antibody
Developmental arteries and neural crest EGF-like protein antibody
EVEC antibody
Fbln5 antibody
FBLN5_HUMAN antibody
FIBL 5 antibody
ExpandImages
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ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
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Immunocytochemistry analysis of Hela cells labeling Fibulin 5 with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution. -
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution.
Lane 1: Rat testis tissue lysate
Lysates/proteins at 40 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50.2 kDa
Observed band size: 50 kDa -
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution.
Lane 1: SK-OV-3 (human ovarian cancer cells) cell tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50.2 kDa
Observed band size: 50 kDa -
Western blot analysis of Fibulin 5 on different lysates with Rabbit anti-Fibulin 5 antibody (ER1802-95) at 1/1,000 dilution.
Lane 1: JAR (human choriocarcinoma cells) cell tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1802-95, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50.2 kDa
Observed band size: 50 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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