Fibulin 5 Mouse Monoclonal Antibody [A1C12]
Catalog# HA600044
Fibulin 5 Mouse Monoclonal Antibody [A1C12]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
Fibulin 5 Mouse Monoclonal Antibody [A1C12]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within human Fibulin 5 aa 200-400.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
50 kDa (Predicted band size)
Positive Control
A549 cell lysate, SKOV-3 cell lysate, Hela cell lysate, SiHa cell lysate, SiHa.
Conjugation
unconjugated
Clone Number
A1C12
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:50-1:100
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FC
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1:100-1:500
Target
Function
Fibulin-5 (also known as DANCE (developmental arteries and neural crest epidermal growth factor (EGF)-like)) is a protein that in humans is encoded by the FBLN5 gene. The protein encoded by this gene is a secreted, extracellular matrix protein containing an Arg-Gly-Asp (RGD) motif and calcium-binding EGF-like domains. It promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. It is prominently expressed in developing arteries but less so in adult vessels. However, its expression is reinduced in balloon-injured vessels and atherosclerotic lesions, notably in intimal vascular smooth muscle cells and endothelial cells. Therefore, the protein encoded by this gene may play a role in vascular development and remodeling. FBLN5 has been shown to interact with LOXL1 and apolipoprotein(a). FBLN5 mutations have been described in patients with age-related macular degeneration, as well as being involved in Charcot-Marie-Tooth neuropathies.
Background References
1. Tekedereli I. et. al. Autosomal recessive cutis laxa: a novel mutation in the FBLN5 gene in a family. Clin Dysmorphol. 2019 Apr;28(2):63-65.
2. Manders DB. et. al. Dysregulation of fibulin-5 and matrix metalloproteases in epithelial ovarian cancer. Oncotarget. 2018 Feb 14;9(18):14251-14267.
Subcellular Location
Secreted, extracellular matrix.
UNIPROT
Synonyms
ADCL2 antibody
ARCL1A antibody
ARMD3 antibody
Dance antibody
Developmental arteries and neural crest EGF like protein antibody
Developmental arteries and neural crest EGF-like protein antibody
EVEC antibody
Fbln5 antibody
FBLN5_HUMAN antibody
FIBL 5 antibody
ExpandImages
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Western blot analysis of Fibulin 5 on different lysates with Mouse anti-Fibulin 5 antibody (HA600044) at 1/1,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 2: SK-OV-3 (Human ovarian cancer cell) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 4: SiHa (Human cervical cancersSquamous cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 6 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA600044, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50 kDa
Observed band size: 110 kDa(Homodimer) -
ICC staining of Fibulin 5 in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA600044, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Flow cytometric analysis of Fibulin 5 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (HA600044, 1ug/ml) (red) compared with Mouse IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at +4℃ for 1 hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃ (dark incubation).Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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