Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
Catalog# HA722532
Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
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WB
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IHC-P
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Mouse
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Rat
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Human
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unconjugated
Overview
Product Name
Follistatin Recombinant Rabbit Monoclonal Antibody [JE31-78]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Follistatin aa 1-150 / 344.
Species Reactivity
Mouse, Rat (Predicted: Human)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 38 kDa
Positive Control
C6 cell lysate, Mouse ovary tissue lysate, Rat ovary tissue lysate, mouse ovary tissue, rat ovary tissue.
Conjugation
unconjugated
Clone Number
JE31-78
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | |
|---|---|---|
| human |
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| mouse |
|
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
Target
Function
Follistatin, also known as activin-bindings protein, is a protein that in humans is encoded by the FST gene. Follistatin is an autocrine glycoprotein that is expressed in nearly all tissues of higher animals. Its primary function is the binding and bioneutralization of members of the TGF-β superfamily, with a particular focus on activin, a paracrine hormone. An earlier name for the same protein was FSH-suppressing protein (FSP). At the time of its initial isolation from follicular fluid, it was found to inhibit the anterior pituitary's secretion of follicle-stimulating hormone (FSH).
Background References
1. Bielka W et al. Follistatin and follistatin-like 3 in metabolic disorders. Prostaglandins Other Lipid Mediat. 2023 Dec
2. Li XJ et al. Follistatin promotes LIN28B-mediated supporting cell reprogramming and hair cell regeneration in the murine cochlea. Sci Adv. 2022 Feb
Subcellular Location
Secreted.
Synonyms
Activin binding protein antibody
Activin-binding protein antibody
Follistatin antibody
FS antibody
fst antibody
FST_HUMAN antibody
Human follistatin gene antibody
Images
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Western blot analysis of Follistatin on different lysates with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
Lane 1: C6 cell lysate (20 µg/Lane)
Lane 2: Mouse ovary tissue lysate (30 µg/Lane)
Lane 3: Rat ovary tissue lysate (30 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722532) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat ovary tissue with Rabbit anti-Follistatin antibody (HA722532) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722532) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Conjugate: unconjugated
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Reactivity: Human,Cynomolgus monkey
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