Biotin Conjugated Human/Monkey Follistatin Recombinant Rabbit Monoclonal Antibody [PSH28-51] - Detector

Sandwich ELISA analysis of Human Follistatin protein matched pair antibodies

Capture: HA724788, Human/Monkey Follistatin Rabbit mAb [PSH28-50]
Detector: HA724789, Human/Monkey Follistatin Rabbit mAb [PSH28-51]

Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724788) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Follistatin protein (HA211463) starting from 8,000 pg/mL to 0 pg/mL and detect antibody (HA724789, Biotin, 0.1 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

Interpolated concentrations of native Follistatin in HepG2 cell extract samples based on a 1,000 µg/mL extract load.

Capture: HA724788, Human/Monkey Follistatin Rabbit mAb [PSH28-50]
Detector: HA724789, Human/Monkey Follistatin Rabbit mAb [PSH28-51]

Interpolated concentration of native Follistatin was measured in duplicate at different sample concentrations. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Follistatin concentration was determined to be 3,611 pg/mL in HepG2 cell extract.

Interpolated concentrations of native Follistatin in human serum.

Capture: HA724788, Human/Monkey Follistatin Rabbit mAb [PSH28-50]
Detector: HA724789, Human/Monkey Follistatin Rabbit mAb [PSH28-51]

Interpolated concentration of native Follistatin was measured in duplicate at different sample concentrations and interpolated from the Follistatin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Follistatin concentration was determined to be 510 pg/mL in human serum.

Interpolated concentrations of native Follistatin in cynomolgus monkey serum.

Capture: HA724788, Human/Monkey Follistatin Rabbit mAb [PSH28-50]
Detector: HA724789, Human/Monkey Follistatin Rabbit mAb [PSH28-51]

Interpolated concentration of native Follistatin was measured in duplicate at different sample concentrations and interpolated from the Follistatin standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Follistatin concentration was determined to be 1,950 pg/mL in cynomolgus monkey serum.

Interpolated concentrations of spiked Follistatin in cell culture media samples.

Capture: HA724788, Human/Monkey Follistatin Rabbit mAb [PSH28-50]
Detector: HA724789, Human/Monkey Follistatin Rabbit mAb [PSH28-51]

The concentrations of Follistatin were measured in duplicates, interpolated from the Follistatin standard curves and corrected for sample dilution. Diluted samples are as follows: 50% cell culture media with FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).