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GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]

Usd: 385

Specification

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Catalog# HA722898

GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human GABRB1 aa 351-400 / 474.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 54 kDa

Positive Control

Human brain tissue lysate, Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE33-35

Reactivity Data

WB
Human
Tested Tested
Mouse
Tested Tested
Rat
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.

Background References

1. Hernandez CC et al. Epileptic Encephalopathy GABRB Structural Variants Share Common Gating and Trafficking Defects. Biomolecules. 2023 Dec.

2. Burgess R et al. The Genetic Landscape of Epilepsy of Infancy with Migrating Focal Seizures. Ann Neurol. 2019 Dec.

Subcellular Location

Postsynaptic cell membrane, Cell membrane.

UNIPROT

SwissProt: P18505 Human

SwissProt: P50571 Mouse

SwissProt: P15431 Rat

Synonyms

AW061132 antibody

B230208N19Rik antibody

GABA(A) receptor beta 1 antibody

GABA(A) receptor subunit beta-1 antibody

GABA-A receptor, beta-1 polypeptide antibody

Gabrb-1 antibody

GABRB1 antibody

Gamma aminobutyric acid (GABA) A receptor beta 1 antibody

Gamma Aminobutyric Acid A Receptor Beta 1 antibody

Gamma Aminobutyric Acid Receptor , beta-1 antibody

Expand

Images

  • Western blot analysis of GABRB1 on different lysates with Rabbit anti-GABRB1 antibody (<a href="/products/HA722898" style="font-weight: bold;text-decoration: underline;">HA722898</a>) at 1/1,000 dilution.<br /><br />Lane 1: Human brain tissue lysate<br />Lane 2: Mouse cerebellum tissue lysate<br />Lane 3: Mouse brain tissue lysate<br />Lane 4: Rat cerebellum tissue lysate<br />Lane 5: Rat brain tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722898" style="font-weight: bold;text-decoration: underline;">HA722898</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GABRB1 on different lysates with Rabbit anti-GABRB1 antibody (HA722898) at 1/1,000 dilution.

    Lane 1: Human brain tissue lysate
    Lane 2: Mouse cerebellum tissue lysate
    Lane 3: Mouse brain tissue lysate
    Lane 4: Rat cerebellum tissue lysate
    Lane 5: Rat brain tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722898) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"