GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]
Catalog# HA722898
GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GABRB1 Recombinant Rabbit Monoclonal Antibody [JE33-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human GABRB1 aa 351-400 / 474.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 54 kDa
Positive Control
Human brain tissue lysate, Mouse cerebellum tissue lysate, Mouse brain tissue lysate, Rat cerebellum tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE33-35
Reactivity Data
| WB | |
|---|---|
| Human |
|
| Mouse |
|
| Rat |
|
Product Features
Form
Liquid
Concentration
1mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.
Background References
1. Hernandez CC et al. Epileptic Encephalopathy GABRB Structural Variants Share Common Gating and Trafficking Defects. Biomolecules. 2023 Dec.
2. Burgess R et al. The Genetic Landscape of Epilepsy of Infancy with Migrating Focal Seizures. Ann Neurol. 2019 Dec.
Subcellular Location
Postsynaptic cell membrane, Cell membrane.
Synonyms
AW061132 antibody
B230208N19Rik antibody
GABA(A) receptor beta 1 antibody
GABA(A) receptor subunit beta-1 antibody
GABA-A receptor, beta-1 polypeptide antibody
Gabrb-1 antibody
GABRB1 antibody
Gamma aminobutyric acid (GABA) A receptor beta 1 antibody
Gamma Aminobutyric Acid A Receptor Beta 1 antibody
Gamma Aminobutyric Acid Receptor , beta-1 antibody
ExpandImages
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Western blot analysis of GABRB1 on different lysates with Rabbit anti-GABRB1 antibody (HA722898) at 1/1,000 dilution.
Lane 1: Human brain tissue lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat cerebellum tissue lysate
Lane 5: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722898) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"