GCSF Receptor Rabbit Polyclonal Antibody

Western blot analysis of GCSF Receptor on HL-60 cell lysate with Rabbit anti-GCSF Receptor antibody (HA500282) at 1/500 dilution.


Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minutes; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500282, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 92 kDa
Observed band size: 100 kDa

ICC staining of GCSF Receptor in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500282, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

Flow cytometric analysis of THP-1 cells labeling GCSF Receptor.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA500282, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).