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GCSF Receptor Recombinant Rabbit Monoclonal Antibody [PSH03-44] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750878

GCSF Receptor Recombinant Rabbit Monoclonal Antibody [PSH03-44] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

GCSF Receptor Recombinant Rabbit Monoclonal Antibody [PSH03-44] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GCSF Receptor aa 651-836 / 836.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 92 kDa

Positive Control

A431 cell lysate, K-562 cell lysate, THP-1 cell lysate, Jurkat cell lysate, Ramos cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, mouse testis tissue lysate, rat placenta tissue lysate, rat brain tissue lysate, rat testis tissue lysate, rat skin tissue lysate.

Conjugation

unconjugated

Clone Number

PSH03-44

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

The granulocyte colony-stimulating factor receptor (G-CSF-R) also known as CD114 (Cluster of Differentiation 114) is a protein that in humans is encoded by the CSF3R gene. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF). The G-CSF receptors belong to a family of cytokine receptors known as the hematopoietin receptor family. The granulocyte colony-stimulating factor receptor is present on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. The G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, and Erk1/2.

Background References

1. Zhao H et al. CSF3R and SETBP1 getting high on LSD1. Blood. 2022 Aug

2. Mohamed A et al. CSF3R mutated myeloid neoplasms: Beyond chronic neutrophilic leukemia. Hum Pathol. 2024 Jul

Subcellular Location

Cell membrane; Secreted.

UNIPROT

SwissProt: Q99062 Human

SwissProt: P40223 Mouse

Entrez Gene: 298518 Rat

Synonyms

CD 114 antibody

CD114 antibody

CD114 antigen antibody

Colony stimulating factor 3 receptor (granulocyte) antibody

Colony stimulating factor 3 receptor antibody

CSF 3R antibody

CSF3R antibody

CSF3R_HUMAN antibody

Csfgr antibody

G CSF R antibody

Expand

Images

  • This data was developed using <a href="/products/HA721998" style="font-weight: bold;text-decoration: underline;">HA721998</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of GCSF Receptor on different lysates with Rabbit anti-GCSF Receptor antibody (<a href="/products/HA721998" style="font-weight: bold;text-decoration: underline;">HA721998</a>) at 1/2,000 dilution.<br /><br />Lane 1: A431 cell lysate (20 µg/Lane)<br />Lane 2: K-562 cell lysate (20 µg/Lane)<br />Lane 3: THP-1 cell lysate (no heat) (20 µg/Lane)<br />Lane 4: Jurkat cell lysate (20 µg/Lane)<br />Lane 5: Ramos cell lysate (20 µg/Lane)<br />Lane 6: Mouse placenta tissue lysate (40 µg/Lane)<br />Lane 7: Mouse brain tissue lysate (no heat) (40 µg/Lane)<br />Lane 8: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 9: Rat placenta tissue lysate (40 µg/Lane)<br />Lane 10: Rat brain tissue lysate (no heat) (40 µg/Lane)<br />Lane 11: Rat testis tissue lysate (40 µg/Lane)<br />Lane 12: Rat skin tissue lysate (no heat) (40 µg/Lane)<br /><br />Notice: no heat means the lysate is not boiled.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 50-110 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721998" style="font-weight: bold;text-decoration: underline;">HA721998</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA721998, the same antibody clone in a different buffer formulation.
    Western blot analysis of GCSF Receptor on different lysates with Rabbit anti-GCSF Receptor antibody (HA721998) at 1/2,000 dilution.

    Lane 1: A431 cell lysate (20 µg/Lane)
    Lane 2: K-562 cell lysate (20 µg/Lane)
    Lane 3: THP-1 cell lysate (no heat) (20 µg/Lane)
    Lane 4: Jurkat cell lysate (20 µg/Lane)
    Lane 5: Ramos cell lysate (20 µg/Lane)
    Lane 6: Mouse placenta tissue lysate (40 µg/Lane)
    Lane 7: Mouse brain tissue lysate (no heat) (40 µg/Lane)
    Lane 8: Mouse testis tissue lysate (40 µg/Lane)
    Lane 9: Rat placenta tissue lysate (40 µg/Lane)
    Lane 10: Rat brain tissue lysate (no heat) (40 µg/Lane)
    Lane 11: Rat testis tissue lysate (40 µg/Lane)
    Lane 12: Rat skin tissue lysate (no heat) (40 µg/Lane)

    Notice: no heat means the lysate is not boiled.

    Predicted band size: 92 kDa
    Observed band size: 50-110 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721998) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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