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Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]

Usd: 385

Specification

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Catalog# ET1702-59

Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GLP1 aa 65-150.

Product Specificity

Glucagon Recombinant Rabbit Monoclonal Antibody [JF33-10] recognizes endogenous levels of GLP-1 and total glucagon protein.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 21 kDa

Positive Control

human pancreas tissue, PC-12, SW480.

Conjugation

unconjugated

Clone Number

JF33-10

RRID

AB_3070321

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:100

  • IHC-P

  • 1:1,000

Target

Function

Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the Glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.

Background References

1. Takada Y et al. Brg1 plays an essential role in development and homeostasis of the duodenum through regulation of Notch signaling. Development 143:3532-3539 (2016).

2. Jiang S et al. AMPK-dependent regulation of GLP1 expression in L-like cells. J Mol Endocrinol 57:151-60 (2016).

Sequence Similarity

Belongs to the glucagon family.

Tissue Specificity

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

Post-translational Modification

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01275 Human

SwissProt: P55095 Mouse

SwissProt: P06883 Rat

Synonyms

GCG antibody

glicentin-related polypeptide antibody

GLP-1 antibody

GLP-1(7-36) antibody

GLP-1(7-37) antibody

GLP-2 antibody

GLP1 antibody

GLP1, included antibody

GLP2 antibody

GLP2, included antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Glucagon with Rabbit anti-Glucagon antibody (<a href="/products/ET1702-59" style="font-weight: bold;text-decoration: underline;">ET1702-59</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/ET1702-59" style="font-weight: bold;text-decoration: underline;">ET1702-59</a>, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Glucagon with Rabbit anti-Glucagon antibody (ET1702-59) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1702-59, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Glucagon antibody (<a href="/products/ET1702-59" style="font-weight: bold;text-decoration: underline;">ET1702-59</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-59" style="font-weight: bold;text-decoration: underline;">ET1702-59</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Glucagon antibody (ET1702-59) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-59) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • ICC staining GLP1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GLP1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining GLP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining GLP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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