Glucagon Mouse Monoclonal Antibody
Catalog# EM1701-34
Glucagon Mouse Monoclonal Antibody
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IHC
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IF-Cell
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Glucagon Mouse Monoclonal Antibody
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant full length human glucagon protein.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IHC, IF-Cell
Molecular Weight
Predicted band size: 21 kDa
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
-15℃ to -25℃/1 year(Do not lower than -25℃)
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Immunogen affinity purified.
Application Dilution
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IHC
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1:200-1:400
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IF-cell
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1:50-1:200
Target
Function
Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.
Background References
1. Furukawa S et al. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol 30:79-107 (2017).
2. Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016).
Sequence Similarity
Belongs to the glucagon family.
Tissue Specificity
Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
Post-translational Modification
Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
Subcellular Location
Secreted.
Synonyms
GCG antibody
Images
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreas. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Glucagon Recombinant Rabbit Monoclonal Antibody [JF0960]
Application: WB,IHC-P,mIHC,IHC-Fr,IF-Tissue
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
