GM-CSF Mouse Monoclonal Antibody [A6C6]
Catalog# HA601032
GM-CSF Mouse Monoclonal Antibody [A6C6]
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WB
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ELISA
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Human
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unconjugated
Overview
Product Name
GM-CSF Mouse Monoclonal Antibody [A6C6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Recombinant protein within Human GM-CSF aa 1-144/144.
Species Reactivity
Human
Validated Applications
WB, ELISA
Target Molecular Weight
Predicted band size: 16 kDa
Conjugation
unconjugated
Clone Number
A6C6
RRID
Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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WB
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1:2,000
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ELISA
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1:30,000
Target
Function
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. Unlike granulocyte colony-stimulating factor, which specifically promotes neutrophil proliferation and maturation, GM-CSF affects more cell types, especially macrophages and eosinophils. GM-CSF is found in high levels in joints with rheumatoid arthritis and blocking GM-CSF as a biological target may reduce the inflammation or damage. Some drugs (e.g. otilimab) are being developed to block GM-CSF. In critically ill patients GM-CSF has been trialled as a therapy for the immunosuppression of critical illness, and has shown promise restoring monocyte and neutrophil function, although the impact on patient outcomes is currently unclear and awaits larger studies. GM-CSF stimulates monocytes and macrophages to produce pro-inflammatory cytokines, including CCL17. Elevated GM-CSF has been shown to contribute to inflammation in inflammatory arthritis, osteoarthritis, colitis asthma, obesity, and COVID-19.
Background References
1. Becher B. et. al. GM-CSF: From Growth Factor to Central Mediator of Tissue Inflammation. Immunity. 2016 Nov
2. Avci AB. et. al. Targeting GM-CSF in rheumatoid arthritis. Clin Exp Rheumatol. 2016 Jul-Aug
Subcellular Location
Secreted.
UNIPROT
Synonyms
Colony stimulating factor 2 (granulocyte-macrophage) antibody
Colony Stimulating Factor 2 antibody
Colony stimulating factor antibody
Colony-stimulating factor antibody
CSF 2 antibody
CSF antibody
CSF2 antibody
CSF2_HUMAN antibody
GM-CSF antibody
GMCSF antibody
ExpandImages
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GM-CSF Antibody (HA601032) in indirect ELISA.
Indirect ELISA analysis of GM-CSF was performed by coating wells of a 96-well plate with 50 µl per well of GM-CSF antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse GM-CSF monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
Western blot analysis of GM-CSF on GM-CSF Recombinant Protein with Mouse anti-GM-CSF antibody (HA601032) at 1/2,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 7 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA601032, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 30 kDa
Observed band size: 30 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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