GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26]
Catalog# HA724519
GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26]
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WB
-
IHC-P
-
IP
-
Human
-
Mouse
-
Rat
-
Green monkey
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unconjugated
Overview
Product Name
GPI Recombinant Rabbit Monoclonal Antibody [PSH25-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GPI aa 2-558.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 63 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, HCT 116 (Human colon cancer cell) cell lysate, PC-3 (Human prostate cancer cell) cell lysate, HEK-293 (Human embryonic kidney cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse liver tissue lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH25-26
Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:500-1:2,000
-
IP
-
1-2μg/sample
Target
Function
Glucose-6-phosphate isomerase (GPI), alternatively known as phosphoglucose isomerase/phosphoglucoisomerase (PGI) or phosphohexose isomerase (PHI), is an enzyme ( EC 5.3.1.9) that in humans is encoded by the GPI gene on chromosome 19. This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate (G6P) and fructose-6-phosphate (F6P). Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor (AMF) based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia, and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
Background References
1. Hong Z et al. Esculetin inhibits liver cancer by targeting glucose-6-phosphate isomerase mediated glycolysis. Biomed Pharmacother. 2025 Jul
2. Gruda Sussman R et al. Glucose 6 Phosphate Isomerase Deficiency, a Rare Hemolytic Anemia Misdiagnosed as Hereditary Spherocytosis. J Pediatr Hematol Oncol. 2023 Jan
Subcellular Location
Cytoplasm, Secreted.
Synonyms
AMF antibody
Aurocrine motility factor antibody
Autocrine motility factor antibody
DKFZp686C13233 antibody
EC 5.3.1.9 antibody
G6PI_HUMAN antibody
Glucose phosphate isomerase antibody
Glucose-6-phosphate isomerase antibody
GNPI antibody
GPI antibody
ExpandImages
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Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA724519) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: HCT 116 (Human colon cancer cell) cell lysate
Lane 3: PC-3 (Human prostate cancer cell) cell lysate
Lane 4: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 5: A549 (Human lung adenocarcinoma cell) cell lysate
Lane 6: COS-1 (African green monkey kidney fibroblast) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 55 kDa -
Western blot analysis of GPI on different lysates with Rabbit anti-GPI antibody (HA724519) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat liver tissue lysate
Lane 5: Rat spleen tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 63 kDa
Observed band size: 55 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724519, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Kindey
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724519, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724519, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Kindey
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724519, 1/2,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724519, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Immunoprecipitation (IP)
GPI was immunoprecipitated in 0.2 mg HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate with HA724519 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA724519 at 1/5,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA724519 IP in HeLa cell lysate
Lane 3: Rabbit IgG instead of HA724519 in HeLa cell lysate
Exposure time: 3 seconds
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary dilution: HA724519, 1/5,000 in primary antibody dilution buffer (K1803), 2 hours at room temperature
Predicted band size: 63 kDa
Observed band size: 55 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"