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Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Mouse IgG1 (Chimeric)

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Specification

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Catalog# HA601621

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Mouse IgG1 (Chimeric)

Application

  • WB

  • IHC-Fr

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Glucose Transporter GLUT1 Recombinant Antibody [SA0377] - Mouse IgG1 (Chimeric)

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Synthetic peptide within Human GLUT1 aa 443-492 / 492.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-Fr, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 54 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cells) (no heat) cell lysates, mouse brain (no heat) tissue lysates.

Conjugation

unconjugated

Clone Number

SA0377

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-Fr

  • 1:500

  • IHC-P

  • 1:5,000

  • IF-Cell

  • 1:100

Target

Function

Glucose transporter 1 (or GLUT1), also known as solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1), is a uniporter protein that in humans is encoded by the SLC2A1 gene. GLUT1 facilitates the transport of glucose across the plasma membranes of mammalian cells. This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. One good source of GLUT1 is erythrocyte membranes. GLUT1 accounts for 2 percent of the protein in the plasma membrane of erythrocytes. GLUT1, found in the plasma membrane of erythrocytes, is a classic example of a uniporter. After glucose is transported into the erythrocyte, it is rapidly phosphorylated, forming glucose-6-phosphate, which cannot leave the cell. Mutations in this gene can cause GLUT1 deficiency syndrome 1, GLUT1 deficiency syndrome 2, idiopathic generalized epilepsy 12, dystonia 9, and stomatin-deficient cryohydrocytosis.

Background References

1. Boyer-Di Ponio J et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS One 9:e84179 (2014).

2. Saucillo DC et al. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192:136-44 (2014).

Subcellular Location

Cell membrane, Melanosome

UNIPROT

SwissProt: P11166 Human

SwissProt: P17809 Mouse

SwissProt: P11167 Rat

Synonyms

Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody

CSE antibody

DYT17 antibody

DYT18 antibody

DYT9 antibody

EIG12 antibody

erythrocyte/brain antibody

Erythrocyte/hepatoma glucose transporter antibody

facilitated glucose transporter member 1 antibody

Glucose transporter 1 antibody

Expand

Images

  • Western blot analysis of Glucose Transporter GLUT1 on HeLa (Human cervical adenocarcinoma cells) (no heat) cell lysate with Mouse anti-Glucose Transporter GLUT1 antibody (<a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>) at 1/50,000 dilution. <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 6 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/50,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 54 kDa <br />Observed band size: 42-55 kDa

    Western blot analysis of Glucose Transporter GLUT1 on HeLa (Human cervical adenocarcinoma cells) (no heat) cell lysate with Mouse anti-Glucose Transporter GLUT1 antibody (HA601621) at 1/50,000 dilution.

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601621, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 54 kDa
    Observed band size: 42-55 kDa

  • Western blot analysis of Glucose Transporter GLUT1 on mouse brain (no heat) tissue lysate with Mouse anti-Glucose Transporter GLUT1 antibody (<a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>) at 1/50,000 dilution. <br /> <br />Lysates/proteins at 10 µg/Lane. <br />Exposure time: 6 seconds; ECL: K1801 <br /> <br />Blocking: 5% NFDM/TBST, 1 hour at room temperature <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/50,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 °C <br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature <br /> <br />Predicted band size: 54 kDa <br />Observed band size: 42-55 kDa

    Western blot analysis of Glucose Transporter GLUT1 on mouse brain (no heat) tissue lysate with Mouse anti-Glucose Transporter GLUT1 antibody (HA601621) at 1/50,000 dilution.

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA601621, 1/50,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 54 kDa
    Observed band size: 42-55 kDa

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Mouse <br />Tissue: Brain <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Brain
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601621, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.

  • Application: Immunofluorescence (IHC-Fr) <br /> <br />Species: Rat <br />Tissue: Brain <br />Sample: Frozen section <br /> <br />Antigen retrieval: Not required <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Rat
    Tissue: Brain
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA601621, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Brain <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Brain
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601621, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Liver <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/5,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Liver
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA601621, 1/5,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: HeLa (Human cervix adenocarcinoma epithelial cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br /> <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br />Primary antibody: <a href="/products/HA601621" style="font-weight: bold;text-decoration: underline;">HA601621</a>, 1/100, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 45 minutes at room temperature. <br /> <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: HeLa (Human cervix adenocarcinoma epithelial cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.

    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.
    Primary antibody: HA601621, 1/100, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.

    Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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