Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R]
Catalog# HA601253
Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R]
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
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HA610128
不含抗保成分
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unconjugated
Overview
Product Name
Grp75 Recombinant Mouse Monoclonal Antibody [6-A9-R]
Antibody Type
Recombinant Mouse Monoclonal Antibody
Immunogen
Synthetic peptide within human Grp75 aa 101-150.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 74 kDa
Positive Control
HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, K-562, human liver tissue.
Conjugation
unconjugated
Clone Number
6-A9-R
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:500
Target
Function
The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.
Background References
1. Burger K et al. Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage. J Cell Biol 216:2373-2389 (2017).
2. Siu GK et al. Hepatitis C virus NS5A protein cooperates with phosphatidylinositol 4-kinase IIIa to induce mitochondrial fragmentation. Sci Rep 6:23464 (2016).
Subcellular Location
Mitochondrion. Nucleus.
Synonyms
75 kDa glucose regulated protein antibody
75 kDa glucose-regulated protein antibody
CSA antibody
Glucose Regulated Protein antibody
Grp 75 antibody
GRP-75 antibody
GRP75 antibody
GRP75_HUMAN antibody
Heat shock 70 kDa protein 9 antibody
Heat shock 70kD protein 9 antibody
ExpandImages
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Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody (HA601253) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Rat kidney tissue lysate
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601253) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of K-562 cells labeling Grp75 with Mouse anti-Grp75 antibody (HA601253) at 1/100 dilution.
Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Grp75 antibody (HA601253) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Grp75 antibody (HA601253) at 1/500 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601253) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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