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HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

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Catalog# HA1023

HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

Application

  • WB

  • ELISA

  • IHC-P

Reactivity

  • Rat

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated Goat anti-Rat IgG(H+L) polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Rat IgG(H+L).

Species Reactivity

Rat

Validated Applications

WB, ELISA, IHC-P

Conjugation

HRP

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:50,000-1:200,000

  • ELISA

  • 1:5,000- 1:10,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

Background References

暂无

Images

  • Western blot analysis of NF-M on different lysates with Rat anti-NF-M antibody (<a href="/products/HA601408" style="font-weight: bold;text-decoration: underline;">HA601408</a>) at 1/5,000 dilution. <br /><br />Lane 1: Mouse brain tissue lysate <br />Lane 2: Mouse liver tissue lysate (negative) <br />Lane 3: Rat brain tissue lysate <br /><br />Lysates/proteins at 20 µg/Lane. <br /><br />Predicted band size: 102 kDa <br />Observed band size: 140 kDa <br /><br />Exposure time: 23 seconds; ECL: K1801; <br />4-20% SDS-PAGE gel. <br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601408" style="font-weight: bold;text-decoration: underline;">HA601408</a>) at 1/50,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rat IgG - HRP Secondary Antibody (<a href="/products/HA1023" style="font-weight: bold;text-decoration: underline;">HA1023</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NF-M on different lysates with Rat anti-NF-M antibody (HA601408) at 1/5,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse liver tissue lysate (negative)
    Lane 3: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 102 kDa
    Observed band size: 140 kDa

    Exposure time: 23 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601408) at 1/50,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rat IgG - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation