Rabbit anti-Guinea pig IgG polyclonal Antibody - BSA and Azide free
Catalog# HA1033
Rabbit anti-Guinea pig IgG polyclonal Antibody - BSA and Azide free
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WB
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ELISA
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Guinea pig
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unconjugated
Overview
Product Name
Rabbit anti-Guinea pig IgG polyclonal Antibody - BSA and Azide free
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Purified native Guinea Pig IgG.
Species Reactivity
Guinea pig
Validated Applications
WB, ELISA
Target Molecular Weight
55/25 kDa
Positive Control
Guinea pig IgG.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
1mg/ml
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4).
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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ELISA
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1:1,000-1:2,000
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Background References
暂无
Images
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Western blot analysis of Rabbit anti-Guinea pig IgG on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA1033, 1/5000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Guinea pig (30 ng per lane)
Lane 2: Mouse IgG (30 ng per lane)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"