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iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Usd: 349

Specification

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Catalog# HA1133

iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Application

  • IF-Cell

  • IF-Tissue

  • FC

  • IHC-Fr

Reactivity

  • Rat

Conjugation

  • iFluor™ 488

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 488 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Rat IgG(H+L).

Species Reactivity

Rat

Validated Applications

IF-Cell, IF-Tissue, FC, IHC-Fr

Conjugation

iFluor™ 488

Reactivity Data

IF-Cell IF-Tissue FC IHC-Fr
Rat
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Mouse
Tested
Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Cell

  • 1:500

  • IF-Tissue

  • 1:500

  • FC

  • 1:500

  • IHC-Fr

  • 1:500

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 SE dye is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Background References

暂无

Images

  • Immunofluorescence analysis of frozen mouse cerebellum tissue with Rat anti-Iba1 antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span><br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601367" style="font-weight: bold;text-decoration: underline;">HA601367</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).<br /><br />Rabbit anti-GLAST /EAAT1 antibody (<a href="/products/ET1704-54" style="font-weight: bold;text-decoration: underline;">ET1704-54</a>, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunofluorescence analysis of frozen mouse cerebellum tissue with Rat anti-Iba1 antibody (HA601367) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601367, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

    Rabbit anti-GLAST /EAAT1 antibody (ET1704-54, red) was stained at 1/1,000 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • Immunofluorescence analysis of frozen mouse hippocampus tissue with Rat anti-Doublecortin/DCX antibody (<a href="/products/HA601398" style="font-weight: bold;text-decoration: underline;">HA601398</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span><br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601398" style="font-weight: bold;text-decoration: underline;">HA601398</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).<br /><br />Rabbit anti-MAP2 antibody (<a href="/products/HA723025" style="font-weight: bold;text-decoration: underline;">HA723025</a>, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunofluorescence analysis of frozen mouse hippocampus tissue with Rat anti-Doublecortin/DCX antibody (HA601398) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601398, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

    Rabbit anti-MAP2 antibody (HA723025, red) was stained at 1/500 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

  • Immunofluorescence analysis of frozen mouse hippocampus tissue with Rat anti-NF-L antibody (<a href="/products/HA601409" style="font-weight: bold;text-decoration: underline;">HA601409</a>) at 1/500 dilution.<br /><br /><span style="font-weight: bold;">The section was not undergone antigen retrieval.</span><br /><br />The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA601409" style="font-weight: bold;text-decoration: underline;">HA601409</a>, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of frozen mouse hippocampus tissue with Rat anti-NF-L antibody (HA601409) at 1/500 dilution.

    The section was not undergone antigen retrieval.

    The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601409, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

  • Immunocytochemistry analysis of C2C12 labeling Nestin with Rat anti-Nestin antibody (<a href="/products/HA601406" style="font-weight: bold;text-decoration: underline;">HA601406</a>) at 1/1,000 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Nestin antibody (<a href="/products/HA601406" style="font-weight: bold;text-decoration: underline;">HA601406</a>) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor&trade; 488, <a href="/products/HA1133" style="font-weight: bold;text-decoration: underline;">HA1133</a>) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of C2C12 labeling Nestin with Rat anti-Nestin antibody (HA601406) at 1/1,000 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-Nestin antibody (HA601406) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation