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iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Usd: 349

Specification

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Catalog# HA1136

iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Application

  • IF-Tissue

  • IHC-Fr

  • IF-Cell

  • FC

Reactivity

  • Rat

Conjugation

  • iFluor™ 594

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 594 Conjugated Goat anti-Rat IgG(H+L) Goat Polyclonal Antibody

Antibody Type

Goat Polyclonal Antibody

Immunogen

Rat IgG(H+L).

Species Reactivity

Rat

Validated Applications

IF-Tissue, IHC-Fr, IF-Cell, FC

Conjugation

iFluor™ 594

Reactivity Data

IF-Tissue IHC-Fr IF-Cell FC
Rat
Tested Tested
Tested Tested
Tested Tested
Tested Tested
Human
Tested

Product Features

Form

Liquid

Concentration

2 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • IF-Tissue

  • 1:500

  • IHC-Fr

  • 1:500

  • IF-Cell

  • 1:500

  • FC

  • 1:500

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Background References

暂无

Images

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Primary Antibody: <a href="/products/HA601414" style="font-weight: bold;text-decoration: underline;">HA601414</a> (1:500)<br /><br />Secondary Antibody Concentration: 1: 500

    Application: IF-Tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Primary Antibody: HA601414 (1:500)

    Secondary Antibody Concentration: 1: 500

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: Cerebellum<br /><br />Sample: Paraffin-embedded section<br /><br />Primary Antibody: <a href="/products/HA601413" style="font-weight: bold;text-decoration: underline;">HA601413</a> (1/1,000)<br /><br />Secondary Antibody Concentration: 1/500

    Application: IF-Tissue

    Species: Mouse

    Site: Cerebellum

    Sample: Paraffin-embedded section

    Primary Antibody: HA601413 (1/1,000)

    Secondary Antibody Concentration: 1/500

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebellum<br />Sample: Frozen section<br /><br />Antigen retrieval: Not required<br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.<br /><br />Primary antibody: <a href="/products/HA601414" style="font-weight: bold;text-decoration: underline;">HA601414</a>, 1/500, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 594, <a href="/products/HA1136" style="font-weight: bold;text-decoration: underline;">HA1136</a>, 1/500), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebellum
    Sample: Frozen section

    Antigen retrieval: Not required

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.

    Primary antibody: HA601414, 1/500, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 1.5 hours at room temperature.

  • Application: Immunocytochemistry (IF-cell)<br /><br />Species: Mouse<br />Sample: C2C12 (Mouse myoblast)<br /><br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.<br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.<br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.<br />Antibody dilution buffer: 1% BSA in PBST.<br /><br />Primary antibody: <a href="/products/HA601406" style="font-weight: bold;text-decoration: underline;">HA601406</a>, 1/1,000, overnight at 4℃.<br />Secondary antibody: Goat Anti-Rat IgG (iFluor&trade; 594, <a href="/products/HA1136" style="font-weight: bold;text-decoration: underline;">HA1136</a>, 1/500), 45 minutes at room temperature.<br />Counterstain: beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Application: Immunocytochemistry (IF-cell)

    Species: Mouse
    Sample: C2C12 (Mouse myoblast)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA601406, 1/1,000, overnight at 4℃.
    Secondary antibody: Goat Anti-Rat IgG (iFluor™ 594, HA1136, 1/500), 45 minutes at room temperature.
    Counterstain: beta Tubulin (ET1602-4, Green), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

Citation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"