iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
Catalog# HA1142
iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
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IHC-Fr
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IF-Tissue
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IF-Cell
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Chicken
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iFluor™ 647
Overview
Product Name
iFluor™ 647 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
Antibody Type
Goat Polyclonal Antibody
Immunogen
Chicken IgY protein.
Species Reactivity
Chicken
Validated Applications
IHC-Fr, IF-Tissue, IF-Cell
Conjugation
iFluor™ 647
Reactivity Data
| IHC-Fr | IF-Tissue | IF-Cell | |
|---|---|---|---|
| Chicken | |||
| Species Independent |
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Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IHC-Fr
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1:1,000
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IF-Tissue
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1:1,000
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IF-Cell
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1:250-1:500
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. iFluor™ 594 dyes have fluorescence excitation and emission maxima of ~590 nm and ~610 nm respectively. iFluor™ 594 family is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family an excellent alternative. iFluor™ 594 is much easier to be conjugated with RPE with much higher conjugation yield, and the resulted RPE-iFluor™ 594 tandem has better FRET efficiency. iFluor™ 594 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
Background References
暂无
Images
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Immunocytochemistry analysis of C6 cells labeling Iba1 with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Chicken anti-Iba1 antibody (HA601376) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Chicken IgY (iFluor™ 647, HA1142) was used as the secondary antibody at 1/250 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"