iFluor™ 750 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
Catalog# HA1145
iFluor™ 750 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
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FC
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IHC-Fr
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IF-Tissue
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IF-Cell
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Chicken
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iFluor™ 750
Overview
Product Name
iFluor™ 750 Conjugated Goat anti-Chicken IgY Goat Polyclonal Antibody
Antibody Type
Goat Polyclonal Antibody
Immunogen
Recombinant protein.
Species Reactivity
Chicken
Validated Applications
FC, IHC-Fr, IF-Tissue, IF-Cell
Conjugation
iFluor™ 750
Reactivity Data
| FC | IHC-Fr | IF-Tissue | IF-Cell | |
|---|---|---|---|---|
| Chicken | ||||
| Species Independent |
|
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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FC
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1:1,000
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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IF-Cell
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1:500
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Background References
暂无
Images
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Flow cytometric analysis of THP-1 cells labeling Iba1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA601376, 1/1,000) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Chicken IgY Secondary antibody (HA1145) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"