iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
Catalog# HA1147
iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
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IHC-Fr
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IF-Tissue
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IF-Cell
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FC
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Guinea pig
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iFluor™ 647
Overview
Product Name
iFluor™ 647 Conjugated Goat anti-Guinea pig IgG Goat Polyclonal Antibody
Antibody Type
Goat Polyclonal Antibody
Immunogen
Guinea pig IgG protein.
Species Reactivity
Guinea pig
Validated Applications
IHC-Fr, IF-Tissue, IF-Cell, FC
Conjugation
iFluor™ 647
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| IHC-Fr | FC | IF-Cell | IF-Tissue | |
|---|---|---|---|---|
| species independent |
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Product Features
Form
Liquid
Concentration
2 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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IHC-Fr
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1:500
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IF-Tissue
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1:500
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IF-Cell
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1:1,000
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FC
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1:200-1:500
Target
Function
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.
Background References
暂无
Images
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Application: Immunofluorescence (IHC-Fr)
Species: Mouse
Tissue: Brain
Sample: Frozen section
Antigen retrieval: Not required
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601368, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature. -
Application: Immunofluorescence (IF-tissue)
Species: Mouse
Tissue: Brain
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃.
Wash buffer: 1× TBST
Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
Primary antibody: HA601368, 1/500, overnight at 4℃.
Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 647, HA1147, 1/500), 1.5 hours at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"