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iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)

Usd: 50

Specification

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Catalog# HA1150

iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)

Application

  • WB

Reactivity

  • Rabbit

Conjugation

  • iFluor™ 680se

Overview

Product Name

iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system)

Antibody Type

Goat Polyclonal Antibody

Immunogen

Rabbit IgG (H+L).

Species Reactivity

Rabbit

Validated Applications

WB

Conjugation

iFluor™ 680se

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB
species independent
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000-1:10,000

Target

Function

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Background References

暂无

Images

  • Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37/90 kDa<br />Observed band size: 37/90 kDa<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor&trade; 680se Secondary Antibody (<a href="/products/HA1150" style="font-weight: bold;text-decoration: underline;">HA1150</a>) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.

    Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate
    Lane 5: C6 cell lysate
    Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37/90 kDa
    Observed band size: 37/90 kDa

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 680se Secondary Antibody (HA1150) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"