ENAH Rabbit Polyclonal Antibody
Catalog# HA500026
ENAH Rabbit Polyclonal Antibody
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WB
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IF-Cell
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ENAH Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human ENAH.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 67 kDa
Positive Control
SiHa cell lysate, Hela cell lysate, 293T cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, MG-63, SiHa.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Cell
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1:200
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FC
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1:500-1:1,000
Target
Function
This gene encodes a member of the enabled/ vasodilator-stimulated phosphoprotein. Members of this gene family are involved in actin-based motility. This protein is involved in regulating the assembly of actin filaments and modulates cell adhesion and motility. Alternate splice variants of this gene have been correlated with tumor invasiveness in certain tissues and these variants may serve as prognostic markers. A pseudogene of this gene is found on chromosome 3.
Background References
1. Wang C. et. al. Circular RNA hsa_circ_0030018 acts as a sponge of miR-599 to aggravate esophageal carcinoma progression by regulating ENAH expression. J Cell Biochem. 2019 Nov
2. Wang DD. et. al. The significance of ENAH in carcinogenesis and prognosis in gastric cancer. Oncotarget. 2017 Aug
Subcellular Location
Cytoskeleton, cytoplasm, lamellipodium, filopodium, synapse, focal adhesion.
Synonyms
ENA antibody
Enah antibody
ENAH_HUMAN antibody
MENA antibody
NDPP1 antibody
Protein enabled homolog antibody
Images
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Western blot analysis of ENAH on different lysates with Rabbit anti-ENAH antibody (HA500026) at 1/1,000 dilution.
Lane 1: NIH/3T3 (Mouse fibroblast) cell lysate
Lane 2: C6 (Rat glioma cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500026, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 66.5 kDa
Observed band size: 67kDa -
Immunocytochemistry analysis of MG-63 cells labeling ENAH with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-ENAH antibody (HA500026) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of SiHa cells labeling ENAH.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA500026, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"