GDF1 Rabbit Polyclonal Antibody
Catalog# HA500058
GDF1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
GDF1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within C-terminal human GDF1.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
SH-SY5Y cell lysate, MCF-7 cell lysate, rat brain tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:100-1:500
Target
Function
Growth differentiation factor 1 (GDF1) is a protein that in humans is encoded by the GDF1 gene. This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a bicistronic mRNA that also encodes the longevity assurance gene.
Background References
1. Gao X. et. al. Association of functional variant in GDF1 promoter with risk of congenital heart disease and its regulation by Nkx2.5. Clin Sci (Lond). 2019 Jun
2. Opazo JC. et. al. Phylogenetic evidence for independent origins of GDF1 and GDF3 genes in anurans and mammals. Sci Rep. 2018 Sep
Subcellular Location
Secreted.
UNIPROT
Synonyms
DORV antibody
DTGA3 antibody
Embryonic growth/differentiation factor 1 antibody
GDF 1 antibody
GDF-1 antibody
GDF1 antibody
GDF1_HUMAN antibody
Growth differentiation factor 1 antibody
Images
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GDF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500058, 1/500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Western blot analysis of GDF1 on different lysates with Rabbit anti-GDF1 antibody (HA500058) at 1/5,000 dilution.
Lane 1: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 100 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500058, 1/5,000 in 5% NFDM/TBST
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 39.5 kDa
Observed band size: 50 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"