TMPRSS2 Rabbit Polyclonal Antibody
Catalog# HA500060
TMPRSS2 Rabbit Polyclonal Antibody
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
TMPRSS2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human TMPRSS2 aa 300-350.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 54 kDa
Positive Control
PC-3M cell lysate, mouse colon tissue lysate, human stomach tissue, human pancreas tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:200-1:1,1000
Target
Function
Plasma membrane-anchored serine protease that participates in proteolytic cascades of relevance for the normal physiologic function of the prostate . Androgen-induced TMPRSS2 activates several substrates that include pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14 leading to extracellular matrix disruption and metastasis of prostate cancer cells . In addition, activates trigeminal neurons and contribute to both spontaneous pain and mechanical allodynia (By similarity).By similarity3 Publications(Microbial infection) Facilitates human coronaviruses SARS-CoV and SARS-CoV-2 infections via two independent mechanisms, proteolytic cleavage of ACE2 receptor which promotes viral uptake, and cleavage of coronavirus spike glycoproteins which activates the glycoprotein for host cell entry . Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.
Background References
1. Wilson S. et. al. The membrane-anchored serine protease, TMPRSS2, activates PAR-2 in prostate cancer cells. Biochem. J. 388:967-972(2005).
Subcellular Location
Cell membrane, Membrane, Secreted.
Synonyms
D16Ertd61e
Epitheliasin
FLJ41954
MGC6821
PP9284
PRSS10
Serine protease 10
TMPRSS2
TMPRSS2 ERG FUSION GENE, INCLUDED
TMPRSS2 ETV1 FUSION GENE, INCLUDED
ExpandImages
-
Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-TMPRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500060, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TMPRSS2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500060, 1/1,500) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
TMPRSS2 Mouse Monoclonal Antibody [A6D9]
Application: WB,IF-Cell
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
Products with the same target and pathway