Strep-Tag II Rabbit Polyclonal Antibody (HA500061)
Catalog# HA500061
Rabbit Polyclonal to Strep-Tag II
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WB
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Species independent
Positive control:
Lane 1: Strep-Tag II protein transfected 293F cells lysate
Lane 2: Non-transfected 293F cells lysate
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Strep-Tag II Rabbit Polyclonal Antibody (HA500061)
Immunogen
Synthetic peptide corresponding to strep-tag ii.
Host
Rabbit
Positive Control
Strep-Tag II protein transfected 293F cells lysate, Non-transfected 293F cells lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:40,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Strep-Tag II
SYNONYMS
anti-Strep-Tag II antibody;Strep-Tag II antibody;Strep-Tag II antibody antibody;Strep-Tag IIantiboy antibody
FUNCTION
The Strep-tag® system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag II is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin®, a specifically engineered streptavidin, and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
CITATIONS
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Zhou, L., Chai, F., He, Y., Zhou, Z., Guo, S., Li, P., Sun, Q., Zu, X., Liu, X., Huang, Q., Zhong, Y., Zhou, A., Wang, X., & Ren, H.
Homodimerized cytoplasmic domain of PD-L1 regulates its complex glycosylation in living cells
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WB
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Human
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Citation
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