Estrogen Sulfotransferase Rabbit Polyclonal Antibody
Catalog# HA500065
Estrogen Sulfotransferase Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Rat
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unconjugated
Overview
Product Name
Estrogen Sulfotransferase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Estrogen Sulfotransferase aa 150-294.
Species Reactivity
Human, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
35 kDa
Positive Control
Human kidney tissue lysates, rat kidney tissue lysates, human small intestine tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
Estrone sulfotransferase (EST) (EC 2.8.2.4), also known as estrogen sulfotransferase, is an enzyme that catalyzes the transformation of an unconjugated estrogen like estrone into a sulfated estrogen like estrone sulfate. It is a steroid sulfotransferase and belongs to the family of transferases, to be specific, the sulfotransferases, which transfer sulfur-containing groups. This enzyme participates in androgen and estrogen metabolism and sulfur metabolism. Steroid sulfatase is an enzyme that catalyzes the reverse reaction, the transfer of a sulfate to an unconjugated estrogen. Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone . Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency . Does not sulfonate cortisol, testosterone and dopamine .
Background References
1. Falany C.N. et. al. Bacterial expression and characterization of a cDNA for human liver estrogen sulfotransferase. J. Steroid Biochem. Mol. Biol. 52:529-539(1995).
Subcellular Location
Cytosol.
Synonyms
EST antibody
EST-1 antibody
EST1 antibody
Estrogen sulfotransferase antibody
estrogen-preferring antibody
estrone sulfotransferase antibody
ST1E1 antibody
ST1E1_HUMAN antibody
STE antibody
Sulfotransferase 1E1 antibody
ExpandImages
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Western blot analysis of Estrogen Sulfotransferase on Mouse kidney tissue lysates with Rabbit anti-Estrogen Sulfotransferase antibody (HA500065) at 1/2,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minute 2 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500065, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 35.1 kDa
Observed band size: 35.1 kDa -
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Estrogen Sulfotransferase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500065, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"