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GABRB3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500090

GABRB3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

GABRB3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human GABRB3 aa 400-443 (Cytoplasmic domain).

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

Rat brain tissue lysate, rat cerebellum tissue lysate, mouse brain tissue lysate, mouse cerebellum tissue lysate, HepG2 cell lysate, 293 cell lysate, rat brain tissue, human lung tissue, mouse brain tissue.

Conjugation

unconjugated

RRID

AB_3071189

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:500

Target

Function

Ligand-gated chloride channel which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. Plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel. The gamma2 subunit is necessary but not sufficient for a rapid formation of active synaptic contacts and the synaptogenic effect of this subunit is influenced by the type of alpha and beta subunits present in the receptor pentamer. The alpha1/beta3/gamma2 receptor exhibits synaptogenic activity. The alpha2/beta3/gamma2 receptor shows very little or no synaptogenic activity. Functions also as histamine receptor and mediates cellular responses to histamine. Plays an important role in somatosensation and in the production of antinociception.

Background References

1. Shi YW. et. al. Synaptic clustering differences due to different GABRB3 mutations cause variable epilepsy syndromes. Brain. 2019 Oct

2. Møller RS. et. al. Mutations in GABRB3: From febrile seizures to epileptic encephalopathies. Neurology. 2017 Jan

Subcellular Location

Plasma membrane, Cell membrane, Cytoplasmic vesicle membrane.

UNIPROT

SwissProt: P28472 Human

SwissProt: P63080 Mouse

SwissProt: P63079 Rat

Synonyms

ECA5 antibody

GABA alpha receptor beta-2 subunit antibody

GABA(A) receptor subunit beta-3 antibody

GABAA receptor beta 3 subunit antibody

GABAA receptor subunit beta 3 antibody

GABR B3 antibody

Gabrb3 antibody

Gamma aminobutyric acid (GABA) A receptor beta 3 antibody

Gamma aminobutyric acid receptor subunit beta 3 antibody

Gamma-aminobutyric acid receptor subunit beta-3 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500090" style="font-weight: bold;text-decoration: underline;">HA500090</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500090" style="font-weight: bold;text-decoration: underline;">HA500090</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500090" style="font-weight: bold;text-decoration: underline;">HA500090</a>, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABRB3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500090, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of GABRB3 on different lysates with Rabbit anti-GABRB3 antibody (<a href="/products/HA500090" style="font-weight: bold;text-decoration: underline;">HA500090</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br /><br /><br />Exposure time: 26 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500090" style="font-weight: bold;text-decoration: underline;">HA500090</a>, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa

    Western blot analysis of GABRB3 on different lysates with Rabbit anti-GABRB3 antibody (HA500090) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate


    Exposure time: 26 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500090, 1/1,000 in 5% NFDM/TBST, 2 hour at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"