RAD52 Rabbit Polyclonal Antibody
Catalog# HA500094
RAD52 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
RAD52 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human RAD52 aa 390-418.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 46 kDa
Positive Control
Hela cell lysate, PC-3M cell lysate, Daudi cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:2,000
Target
Function
The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Background References
1. Jalan M. et. al. Emerging Roles of RAD52 in Genome Maintenance. Cancers (Basel). 2019 Jul
2. Malacaria E. et. al. Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation. Nat Commun. 2019 Mar
Subcellular Location
Nucleus.
UNIPROT
Synonyms
DNA repair protein RAD52 antibody
DNA repair protein RAD52 antibody
DNA repair protein RAD52 homolog antibody
RAD 52 antibody
Rad52 antibody
RAD52 homolog (S. cerevisiae) antibody
RAD52 homolog (S. cerevisiae) antibody
RAD52 homolog antibody
RAD52_HUMAN antibody
Recombination protein RAD52 antibody
ExpandImages
-
Western blot analysis of RAD52 on different lysates with Rabbit anti-RAD52 antibody (HA500094) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HL-60 cell lysate
Lane 3: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500094) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"