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TRAP alpha Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500105

TRAP alpha Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TRAP alpha Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human TRAP alpha aa 19-200.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

32 kDa

Positive Control

A431 cell lysate, HepG2 cell lysate, rat stomach tissue lysate, rat kidney tissue, mouse kidney tissue.

Conjugation

unconjugated

RRID

AB_3071204

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:500-1:2,000

Target

Function

The signal sequence receptor (SSR) is a glycosylated endoplasmic reticulum (ER) membrane receptor associated with protein translocation across the ER membrane. The SSR consists of 2 subunits, a 34-kD glycoprotein encoded by this gene and a 22-kD glycoprotein. This gene generates several mRNA species as a result of complex alternative polyadenylation. This gene is unusual in that it utilizes arrays of polyA signal sequences that are mostly non-canonical. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Zhang M. et. al. A tetratricopeptide repeat domain-containing protein SSR1 located in mitochondria is involved in root development and auxin polar transport in Arabidopsis. Plant J. 2015 Aug

2. Lin YL. et. al. The status of EGFR CA SSR1 is a potential prognostic factor for patients with oral squamous cell carcinoma. Oral Oncol. 2011 Jun

Subcellular Location

Endoplasmic reticulum membrane.

UNIPROT

SwissProt: P43307 Human

SwissProt: Q9CY50 Mouse

SwissProt: Q7TPJ0 Rat

Synonyms

DKFZp781N23103 antibody

FLJ14232 antibody

FLJ22100 antibody

FLJ23034 antibody

FLJ78242 antibody

FLJ93042 antibody

PGP35 antibody

Signal sequence receptor alpha antibody

Signal sequence receptor alpha subunit antibody

Signal sequence receptor subunit alpha antibody

Expand

Images

  • Western blot analysis of TRAP alpha on different lysates with Rabbit anti-TRAP alpha antibody (<a href="/products/HA500105" style="font-weight: bold;text-decoration: underline;">HA500105</a>) at 1/5,000 dilution.<br /><br />Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 2: HL-60 (Human acute promyelocytic leukemia cell) cell lysate<br />Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 4: Rat stomach tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 127 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500105" style="font-weight: bold;text-decoration: underline;">HA500105</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 32 kDa<br />Observed band size: 34 kDa

    Western blot analysis of TRAP alpha on different lysates with Rabbit anti-TRAP alpha antibody (HA500105) at 1/5,000 dilution.

    Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 2: HL-60 (Human acute promyelocytic leukemia cell) cell lysate
    Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 4: Rat stomach tissue lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 127 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500105, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 32 kDa
    Observed band size: 34 kDa

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500105" style="font-weight: bold;text-decoration: underline;">HA500105</a>, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500105, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500105" style="font-weight: bold;text-decoration: underline;">HA500105</a>, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRAP alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500105, 1/1000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"