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Arogenate dehydratase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500108

Arogenate dehydratase Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Rice

  • Arabidopsis thaliana

Conjugation

  • unconjugated

Overview

Product Name

Arogenate dehydratase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within Rice Arogenate dehydratase aa 100-150.

Species Reactivity

Rice, Arabidopsis thaliana

Validated Applications

WB

Molecular Weight

Predicted band size: 46 kDa

Positive Control

Rice tissue lysates, Arabidopsis thaliana tissue lysates.

Conjugation

unconjugated

RRID

AB_3071207

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.

Background References

1. Warpeha K.M. et. al. G-protein-coupled receptor 1, G-protein Galpha-subunit 1, and prephenate dehydratase 1 are required for blue light-induced production of phenylalanine in etiolated Arabidopsis. Plant Physiol. 140:844-855(2006) .

Subcellular Location

Chloroplast, Plastid.

UNIPROT

SwissProt: Q0JDF7 Rice

SwissProt: Q7XLY2 Rice

SwissProt: Q650V6 Rice

SwissProt: Q9ZUY3 ArabidopsisThaliana

Synonyms

Arogenate dehydratase antibody

Images

  • Western blot analysis of Arogenate dehydratase on Rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500108" style="font-weight: bold;text-decoration: underline;">HA500108</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Arogenate dehydratase on Rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500108, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Arogenate dehydratase on Arabidopsis thaliana tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/HA500108" style="font-weight: bold;text-decoration: underline;">HA500108</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Arogenate dehydratase on Arabidopsis thaliana tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500108, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"