MCRS1 Rabbit Polyclonal Antibody
Catalog# HA500120
MCRS1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
MCRS1 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human MCRS1 aa 1-200.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
SH-SY5Y cell lysates, rat large intestine tissue, human liver tissue, human pancreas tissue, mouse testis tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
Target
Function
MSP58, also known as MCRS1 (microspherule protein 1), P78 or INO80Q, is a 462 amino acid protein that localizes to the nucleus and contains one FHA domain. Expressed at high levels during the S phase of the cell cycle and present in testis, prostate, thymus, spleen and colon, MSP58 functions to modulate the transcriptional activity of Daxx (a transcriptional repressor) by recruiting Daxx to the nucleolus. Additionally, MSP58 may play a role in the inhibition of TERT telomerase activity, further implicating MSP58 as an important protein in transcriptional regulation. The gene encoding MSP58 maps to human chromosome 12 and is expressed as multiple alternatively spliced isoforms. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome and is associated wirh hypochondrogenesis, achondrogenesis and Kniest dysplasia.
Background References
1. Wang XM. et. al. Effects of MCRS1 on proliferation, migration, invasion, and epithelial mesenchymal transition of gastric cancer cells by interacting with Pkmyt1 protein kinase. Cell Signal. 2019 Jul
2. Yang H. et. al. Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. Mol Biol Cell. 2019 Apr
Subcellular Location
Nucleus, Nucleolus, Cytoplasm.
Synonyms
58 kDa microspherule protein antibody
Cell cycle regulated factor (78 kDa) antibody
Cell cycle regulated factor p78 antibody
Cell cycle-regulated factor p78 antibody
ICP22BP antibody
INO80 complex subunit J antibody
MCRS 1 antibody
MCRS 2 antibody
MCRS1 antibody
MCRS1_HUMAN antibody
ExpandImages
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Western blot analysis of MCRS1 on SH-SY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500120, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.ne 2: SH-SY5Y cell lysate
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Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MCRS1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500120, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"