Placental lactogen Rabbit Polyclonal Antibody
Catalog# HA500128
Placental lactogen Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
Placental lactogen Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human Placental lactogen aa 50-217.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
25 kDa
Positive Control
Human placenta tissue lysates, human placenta tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:500-1:1,000
Target
Function
The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome.
Background References
1. Cattini PA. et. al. Obesity and regulation of human placental lactogen production in pregnancy. J Neuroendocrinol. 2020 Nov
Subcellular Location
Secreted.
UNIPROT
Synonyms
Choriomammotropin antibody
Chorionic somatomammotropin A antibody
chorionic somatomammotropin B antibody
chorionic somatomammotropin hormone 1 (placental lactogen) antibody
Chorionic somatomammotropin hormone 1 antibody
chorionic somatomammotropin hormone 2 antibody
Chorionic somatomammotropin hormone antibody
CS 2 antibody
CS-2 antibody
CS1 antibody
ExpandImages
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Western blot analysis of Placental lactogen on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500128, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Placental lactogen antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500128, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
Placental lactogen Recombinant Rabbit Monoclonal Antibody [JE60-49]
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
