PPP2R1A Rabbit Polyclonal Antibody
Catalog# HA500160
PPP2R1A Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PPP2R1A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human PPP2R1A aa 100-400.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
65 kDa
Positive Control
Hela cell lysate, 293 cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:100-1:500
Target
Function
This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A.
Background References
1. Zhu D. et. al. Galpha12 directly interacts with PP2A: evidence for Galpha12-stimulated PP2A phosphatase activity and dephosphorylation of microtubule-associated protein, tau. J. Biol. Chem. 279:54983-54986(2004).
Subcellular Location
Cell membrane, Cell projection, Centromere, Chromosome, Cytoplasm, Membrane, Nucleus.
Synonyms
2AAA_HUMAN antibody
6330556D22Rik antibody
Alpha isoform of regulatory subunit A, protein phosphatase antibody
DKFZp470L0914 antibody
Medium tumor antigen-associated 61 kDa protein antibody
MGC128399 antibody
MGC786 antibody
MGC83000 antibody
MGC95083 antibody
PP2A Aalpha antibody
ExpandImages
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Western blot analysis of PPP2R1A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500160, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: 293 cell lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Rat brain tissue lysate -
Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-PPP2R1A antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500160, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"