Elongation factor 1-gamma Rabbit Polyclonal Antibody
Catalog# HA500164
Elongation factor 1-gamma Rabbit Polyclonal Antibody
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Elongation factor 1-gamma Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human EEF1G aa 380-437.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
293 cell lysate, rat testis tissue lysate, mouse brain tissue, human colon tissue, mouse cerebellum tissue, mouse hippocampus tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:5000
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IHC-P
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1:800-1:4,000
Target
Function
Elongation factor 1-gamma is a protein that in humans is encoded by the EEF1G gene.This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Background References
1. Gevaert K. et. al. Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat. Biotechnol. 21:566-569(2003).
Subcellular Location
cytosol, extracellular exosome, nucleus, cytoplasm, membrane.
Synonyms
2610301D06Rik antibody
AA407312 antibody
eEF 1B gamma antibody
EEF 1G antibody
eEF-1B gamma antibody
EEF1G antibody
EF 1 gamma antibody
EF 1G antibody
EF-1-gamma antibody
EF1 gamma antibody
ExpandImages
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Western blot analysis of Elongation factor 1-gamma on different lysates with Rabbit anti-Elongation factor 1-gamma antibody (HA500164) at 1/5,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) cell lysate(15 µg/Lane)
Lane 2: HeLa (Human cervical adenocarcinoma cell) cell lysate(15 µg/Lane)
Lane 3: MCF7 (Human breast cancer cell) cell lysate(15 µg/Lane)
Lane 4: NIH/3T3 (Mouse fibroblast) cell lysate(15 µg/Lane)
Lane 5: mouse testis tissue lysaste(30 µg/Lane)
Lane 6: rat testis tissue lysaste(30 µg/Lane)
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500164, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 50 kDa
Observed band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Elongation factor 1-gamma antibody (HA500164) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500164) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Elongation factor 1-gamma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500164, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Elongation factor 1-gamma antibody (HA500164) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500164) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Elongation factor 1-gamma antibody (HA500164) at 1/4,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500164) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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