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Flightless 1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# HA500167

Flightless 1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Flightless 1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Flightless 1 aa 1100-1269 / 1269.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 145 kDa

Positive Control

HeLa (Human cervical adenocarcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, A549 (Human lung adenocarcinoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, human lung tissue, mouse lung tissue, rat lung tissue.

Conjugation

unconjugated

RRID

AB_3071264

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:500-1:2,000

Target

Function

May play a role as coactivator in transcriptional activation by hormone-activated nuclear receptors (NR) and acts in cooperation with NCOA2 and CARM1. Involved in estrogen hormone signaling. Involved in early embryonic development (By similarity). May play a role in regulation of cytoskeletal rearrangements involved in cytokinesis and cell migration, by inhibiting Rac1-dependent paxillin phosphorylation.

Background References

1. Lee Y.-H. et. al. Developmentally essential protein flightless I is a nuclear receptor coactivator with actin binding activity. Mol. Cell. Biol. 24:2103-2117(2004).

Subcellular Location

Cytoskeleton, centrosome, nucleus, focal adhesion.

UNIPROT

SwissProt: Q13045 Human

SwissProt: Q9JJ28 Mouse

Entrez Gene: 287375 Rat

Synonyms

Fli 1 antibody

FLI antibody

Fli1 antibody

Flightless 1 antibody

Flightless I (Drosophila) homolog antibody

Flightless I homolog antibody

Flightless I homolog (Drosophila) antibody

Flightless1 antibody

FlightlessI antibody

fliI antibody

Expand

Images

  • Western blot analysis of Flightless 1 on different lysates with Rabbit anti-Flightless 1 antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/10,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate<br />Lane 4: MCF7 (Human breast cancer cell) cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 145 kDa<br />Observed band size: 145 kDa

    Western blot analysis of Flightless 1 on different lysates with Rabbit anti-Flightless 1 antibody (HA500167) at 1/10,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 3: A549 (Human lung adenocarcinoma cell) cell lysate
    Lane 4: MCF7 (Human breast cancer cell) cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA500167, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 145 kDa
    Observed band size: 145 kDa

  • Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Flightless 1 antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Flightless 1 antibody (HA500167) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Flightless 1 antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Rabbit anti-Flightless 1 antibody (HA500167) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Flightless 1 antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA500167" style="font-weight: bold;text-decoration: underline;">HA500167</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat lung tissue with Rabbit anti-Flightless 1 antibody (HA500167) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500167) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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